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Cellular profile of the dorsal raphe lateral wing sub-region: Relationship to the lateral dorsal tegmental nucleus
Journal article   Open access   Peer reviewed

Cellular profile of the dorsal raphe lateral wing sub-region: Relationship to the lateral dorsal tegmental nucleus

Rani K. Vasudeva and Barry D. Waterhouse
Journal of chemical neuroanatomy, v 57-58, pp 15-23
May 2014
DOI: https://doi.org/10.1016/j.jchemneu.2014.03.001
PMID: 24704911
Featured in Collection :   UN Sustainable Development Goals @ Drexel
url
https://europepmc.org/articles/pmc4065778View
Accepted (AM)Open Access (License Unspecified),  Open

Abstract

Acetylcholine Dorsal raphe Lateral dorsal tegmental nucleus Nitric oxide synthase Serotonin
•The relationship of NOS cells within the DRN to the lateral dorsal tegmental nucleus (LDT) is examined.•Immunohistochemistry shows DRN lateral wing (LW) NOS cells have 5HT1A receptors and are cholineragic.•LW NOS cells appear to be a rostral extension of the cholinergic LDT and not part of the DRN.•Studies grouping NOS neurons with the DRN LW did not consider the cholinergic content of these cells.•A re-examination of prior physiological and behavioral DRN data with regard to anxiety is needed. As one of the main serotonergic (5HT) projections to the forebrain, the dorsal raphe nucleus (DRN) has been implicated in disorders of anxiety and depression. Although the nucleus contains the densest population of 5HT neurons in the brain, at least 50% of cells within this structure are non-serotonergic, including a large population of nitric oxide synthase (NOS) containing neurons. The DRN has a unique topographical efferent organization and can also be divided into sub-regions based on rostro-caudal and medio-lateral dimensions. NOS is co-localized with 5HT in the midline DRN but NOS-positive cells in the lateral wing (LW) of the nucleus do not express 5HT. Interestingly, the NOS LW neuronal population is immediately rostral to and in line with the cholinergic lateral dorsal tegmental nucleus (LDT). We used immunohistochemical methods to investigate the potential serotonergic regulation of NOS LW neurons and also the association of this cell grouping to the LDT. Our results indicate that >75% of NOS LW neurons express the inhibitory 5HT1A receptor and are cholinergic (>90%). The findings suggest this assembly of cells is a rostral extension of the LDT, one that it is subject to regulation by 5HT release. As such the present study suggests a link between 5HT signaling, activation of cholinergic/NOS neurons, and the stress response including the pathophysiology underlying anxiety and depression.

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Collaboration types
Domestic collaboration
Web of Science research areas
Biochemistry & Molecular Biology
Neurosciences
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