Journal article
Cerebellar Injury Induces NADPH Diaphorase in Purkinje and Inferior Olivary Neurons in the Rat
Experimental neurology, v 126(2)
Apr 1994
PMID: 7925824
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Abstract
NADPH diaphorase (NADPH-D) histochemistry was examined following cerebellar lesions induced by stab or knife cuts. A population of Purkinje cells adjacent to the injury became NADPH-D positive after either type of lesion. These cells appeared by 3 days postlesion and were still seen after 6 weeks. Somata of reactive cells shrank gradually and were reduced about 38% in size by 6 weeks after lesion. In the inferior olive of animals subjected to cerebellar knife cut, NADPH-D-positive cells were found in the beta nucleus, dorsal cap, and subnucleus C. These cells became NADPH-D positive 5 days postlesion, were shrunken at 4 weeks postlesion, and were reduced about 26% in somata size by 6 weeks after lesion. Other cerebellar afferents did not exhibit obvious NADPH-D induction following these lesions. In unlesioned animals, no Purkinje cells or inferior olivary neurons were NADPH-D positive. The present study demonstrates that cerebellar damage induces NADPH-D, a marker for nitric oxide (NO) synthase, in Purkinje cells and inferior olivary neurons. In addition, these reactive neurons exhibit long-term degenerative changes. Taken together with previous studies, these findings indicate that NO, produced by the lesion-induced nitric oxide synthase (NOS), may contribute to the degeneration of brain neurons after injury. The lack of NADPH-D-positive neurons in either Purkinje or the inferior olivary neurons in nonlesioned animals indicates that this may provide a useful model system to study injury-induced NOS in brain.
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Details
- Title
- Cerebellar Injury Induces NADPH Diaphorase in Purkinje and Inferior Olivary Neurons in the Rat
- Creators
- Sheng Chen - Hahnemann University HospitalGary Aston-Jones - Hahnemann University HospitalSongming Chen - Microbiology and Immunology
- Publication Details
- Experimental neurology, v 126(2)
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:A1994NM71600012
- Scopus ID
- 2-s2.0-0028275291
- Other Identifier
- 991019184294004721
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- Web of Science research areas
- Neurosciences