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Published, Version of Record (VoR)Open Access (License Unspecified), Open
Journal article
Characterisation of the efficacy of endodontic medications using a three-dimensional fluorescent tooth model: An ex vivo study
Australian endodontic journal, v 41(2), pp 88-96
Aug 2015
PMID: 25583457
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The purpose of this study was to establish a three-dimensional fluorescent tooth model to investigate bacterial viability against intra-canal medicaments across the thickness and surface of root dentine. Dental microbial biofilms (Enterococcus faecalis and Streptococcus mutans) were established on the external root surface and bacterial kill was monitored over time against intra-canal medicament (Ca(OH)2 ) using fluorescent microscopy in conjunction with BacLight SYTO9 and propidium iodide stains. An Olympus digital camera fitted to SZX16 fluorescent microscope captured images of bacterial cells in biofilms on the external root surface. Viability of biofilm was measured by calculating the total pixel area of green (viable bacteria) and red (non-viable bacteria) for each image using ImageJ® software. All data generated were assessed for normality and then analysed using a Mann-Whitney t-test. The viability of S. mutans biofilm following Ca(OH)2 treatment showed a significant decline compared with the untreated group (P = 0.0418). No significant difference was seen for E. faecalis biofilm between the Ca(OH)2 and untreated groups indicating Ca(OH)2 medicament is ineffective against E. faecalis biofilm. This novel three-dimensional fluorescent biofilm model provides a new clinically relevant tool for testing of medicaments against dental biofilms.
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Details
- Title
- Characterisation of the efficacy of endodontic medications using a three-dimensional fluorescent tooth model: An ex vivo study
- Creators
- Emily W Chen - Griffith UniversityAlison J Carey - Griffith UniversityGlen C Ulett - Griffith UniversityRoy George - Griffith University
- Publication Details
- Australian endodontic journal, v 41(2), pp 88-96
- Publisher
- Wiley
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pediatrics; College of Medicine; Drexel University
- Web of Science ID
- WOS:000358801900004
- Scopus ID
- 2-s2.0-84938208744
- Other Identifier
- 991020099210804721
UN Sustainable Development Goals (SDGs)
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Dentistry, Oral Surgery & Medicine