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Characterization and localization of two ion-binding sites within the pore of cardiac L-type calcium channels
Journal article   Open access   Peer reviewed

Characterization and localization of two ion-binding sites within the pore of cardiac L-type calcium channels

R Rosenberg and Xiao-Hua Chen
The Journal of general physiology, v 97(6), pp 1207-1225
01 Jun 1991
DOI: https://doi.org/10.1085/jgp.97.6.1207
PMID: 1651978
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https://doi.org/10.1085/jgp.97.6.1207View
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Abstract

calcium membrane proteins
L-type Ca channels from porcine cardiac sarcolemma were incorporated into planar lipid bilayers. We characterized interactions of permeant and blocking ions with the channel's pore by (a) studying the current-voltage relationships for Ca super(2+) and Na super(+) when equal concentrations of the ions were present in both internal and external solutions, (b) testing the dose-dependent block of Ba super(2+) currents through the channels by internally applied cadmium, and (c) examining the dose and voltage dependence of the block of Na super(+) currents through the channels by internally and externally applied Ca super(2+). The data provide direct experimental evidence for the existence of at least two ion binding sites with high affinity for Ca super(2+), and support the idea that the sites are symmetrically located within the electric field across L-type Ca channels.

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