Journal article
Characterization and purification of a functional rat glucocorticoid receptor overexpressed in a baculovirus system
The Journal of biological chemistry, v 266(6), pp 3925-3936
25 Feb 1991
PMID: 1995640
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The structure-function relationship of the oligomeric unactivated glucocorticoid receptor is not fully understood. An essential step in the process of understanding such a relationship involves the production of large quantities of the receptor. Using a baculovirus expression system we have been able to overproduce a recombinant rat glucocorticoid receptor (rGR). A cDNA coding for the entire rGR was introduced into the genome of the wild type baculovirus, Autographa californica nuclear polyhedrosis virus, by an in vivo recombination event. Based on specific steroid binding, insect cells infected with the recombinant baculovirus expressed 1-3 x 10 super(6) receptor molecules/cell which is 15-45 times more than that expressed normally in a hepatocyte.
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Details
- Title
- Characterization and purification of a functional rat glucocorticoid receptor overexpressed in a baculovirus system
- Creators
- E Alnemri - Temple UniversityA B Maksymowych - Temple UniversityN Robertson - Temple UniversityG Litwack - Temple University
- Publication Details
- The Journal of biological chemistry, v 266(6), pp 3925-3936
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- SOM Dean - Research Administration
- Web of Science ID
- WOS:A1991EY68200086
- Scopus ID
- 2-s2.0-0025891044
- Other Identifier
- 991021463455604721
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- Web of Science research areas
- Biochemistry & Molecular Biology