Journal article
Characterization of CD31 expression on murine and human neonatal T lymphocytes during development and activation
Pediatric research, v 82(1), pp 133-140
01 Jul 2017
PMID: 28355204
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
BACKGROUND: CD31, expressed by the majority of the neonatal T-cell pool, is involved in modulation of T-cell receptor signaling by increasing the threshold for T-cell activation. Therefore, CD31 could modulate neonatal tolerance and adaptive immune responses.
METHODS: Lymphocytes were harvested from murine neonates at different ages, human late preterm and term cord blood, and adult peripheral blood. Human samples were activated over a 5-day period to simulate acute inflammation. Mice were infected with influenza; lungs and spleens were harvested at days 6 and 9 post infection and analyzed by flow cytometry.
RESULTS: CD31-expressing neonatal murine CD4(+) and CD8a(+) T cells increase over the first week of life. Upon in vitro stimulation, human infants' CD4(+) and CD8a(+) T cells shed CD31 faster in comparison with adults. In the context of acute infection, mice infected at 3 days of age have an increased number of naive and activated CD31(+) T lymphocytes at the site of infection at days 6 and 9 post infection, as compared with those infected at 7 days of age; however, the opposite is true in the periphery.
CONCLUSION: Differences in trafficking of CD31(+) cytotoxic T lymphocytes (CTLs) during acute influenza infection could modulate tolerance and contribute to a dampened adaptive immune response in neonates.
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Details
- Title
- Characterization of CD31 expression on murine and human neonatal T lymphocytes during development and activation
- Creators
- Adam J. Fike - Drexel University, Microbiology and ImmunologyLinda T. Nguyen - Drexel University, PediatricsOgan K. Kumova - Drexel University, Microbiology and ImmunologyAlison J. Carey - Drexel University, Microbiology and Immunology
- Publication Details
- Pediatric research, v 82(1), pp 133-140
- Publisher
- Springer Nature
- Number of pages
- 8
- Grant note
- K08AI108791 / NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Allergy & Infectious Diseases (NIAID) K08AI108791 / National Institute of Allergy and Infectious Diseases of the National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Allergy & Infectious Diseases (NIAID) Margaret Q. Landenberger Foundation
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology; Pediatrics
- Web of Science ID
- WOS:000406256000021
- Scopus ID
- 2-s2.0-85024364224
- Other Identifier
- 991019168484704721
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- Web of Science research areas
- Pediatrics