Journal article
Characterization of New Detergents and Detergent Mimetics by Scattering Techniques for Membrane Protein Crystallization
Methods in molecular biology (Clifton, N.J.), v 1635
2017
PMID: 28755369
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Membrane proteins are difficult to manipulate and stabilize once they have been removed from their native membranes. However, despite these difficulties, successes in membrane-protein structure determination have continued to accumulate for over two decades, thanks to advances in chemistry and technology. Many of these advances have resulted from efforts focused on protein engineering, high-throughput expression, and development of detergent screens, all with the aim of enhancing protein stability for biochemistry and biophysical studies. In contrast, considerably less work has been done to decipher the basic mechanisms that underlie the structure of protein-detergent complexes and to describe the influence of detergent structure on stabilization and crystallization. These questions can be addressed using scattering techniques (employing light, X-rays, and/or neutrons), which are suitable to describe the structure and conformation of macromolecules in solution, as well as to assess weak interactions between particles, both of which are clearly germane to crystallization. These techniques can be used either in batch modes or coupled to size-exclusion chromatography, and offer the potential to describe the conformation of a detergent-solubilized membrane protein and to quantify and model detergent bound to the protein in order to optimize crystal packing. We will describe relevant techniques and present examples of scattering experiments, which allow one to explore interactions between micelles and between membrane protein complexes, and relate these interactions to membrane protein crystallization.
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Details
- Title
- Characterization of New Detergents and Detergent Mimetics by Scattering Techniques for Membrane Protein Crystallization
- Creators
- Françoise Bonneté - University of AvignonPatrick J Loll - Drexel University
- Publication Details
- Methods in molecular biology (Clifton, N.J.), v 1635
- Publisher
- Springer Nature
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:000428500000010
- Scopus ID
- 2-s2.0-85029377344
- Other Identifier
- 1493971514; 9781493971510; 991019170589204721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biochemical Research Methods
- Biochemistry & Molecular Biology