Journal article
Characterization of a Novel Isoform of α-Nascent Polypeptide-associated Complex as IgE-defined Autoantigen
Journal of investigative dermatology, v 119(4), pp 820-829
01 Oct 2002
PMID: 12406326
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The nascent polypeptide-associated complex is required for intracellular translocation of newly synthesized polypeptides in eukaryotic cells. It may also act as a transcriptional coactivator in humans and various eukaryotic organisms and binds to nucleic acids. Recently, we provided evidence that a component of nascent polypeptide-associated complex, α-nascent polypeptide-associated complex, represents an IgE-reactive autoantigen for atopic dermatitis patients. By oligonucleotide screening we isolated a complete cDNA coding for a so far unknown α-nascent polypeptide-associated complex isoform from a human epithelial cDNA library. Southern blot hybridization experiments provided further evidence that α-nascent polypeptide-associated complex is encoded by a gene family. Recombinant α-nascent polypeptide-associated complex was expressed in Escherichia coli as a soluble, His-tagged protein, and purified via nickel affinity chromatography. By circular dichroism analysis it is demonstrated that purified recombinant α-nascent polypeptide-associated complex represents a folded protein of mixed α-helical and β-sheet conformation with unusual high thermal stability and remarkable refolding capacity. Complete recombinant α-nascent polypeptide-associated complex (215 amino acids) and its 86 amino acid C-terminal fragment specifically bound IgE autoantibodies. Recombinant α-nascent polypeptide-associated complex also inhibited IgE binding to natural α-nascent polypeptide-associated complex, demonstrating the presence of common IgE epitopes between the recombinant and natural protein. Furthermore, recombinant α-nascent polypeptide-associated complex induced specific lymphoproliferative responses in peripheral blood mononuclear cells of a sensitized atopic dermatitis patient. As has been proposed for environmental allergens it is possible that T cell responses to IgE-defined autoantigens may contribute to the chronic skin manifestations in atopic dermatitis.
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Details
- Title
- Characterization of a Novel Isoform of α-Nascent Polypeptide-associated Complex as IgE-defined Autoantigen
- Creators
- Roschanak Mossabeb - University of ViennaSusanne Seiberler - University of ViennaIrene Mittermann - University of ViennaSusanne Natter - University of ViennaDietrich Kraft - University of GrazRudolf Valenta - University of GrazRenate Reininger - University of ViennaSusanne Spitzauer - University of ViennaPetra Verdino - University of ViennaWalter Keller - University of Vienna
- Publication Details
- Journal of investigative dermatology, v 119(4), pp 820-829
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pediatrics
- Web of Science ID
- WOS:000179072800009
- Scopus ID
- 2-s2.0-18744394320
- Other Identifier
- 991021838263204721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Dermatology