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Journal article
Characterization of a Plasmodium falciparum Orthologue of the Yeast Ubiquinone-Binding Protein, Coq10p
PloS one, v 11(3), pp e0152197-e0152197
2016
PMID: 27015086
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Coenzyme Q (CoQ, ubiquinone) is a central electron carrier in mitochondrial respiration. CoQ is synthesized through multiple steps involving a number of different enzymes. The prevailing view that the CoQ used in respiration exists as a free pool that diffuses throughout the mitochondrial inner membrane bilayer has recently been challenged. In the yeast Saccharomyces cerevisiae, deletion of the gene encoding Coq10p results in respiration deficiency without inhibiting the synthesis of CoQ, suggesting that the Coq10 protein is critical for the delivery of CoQ to the site(s) of respiration. The precise mechanism by which this is achieved remains unknown at present. We have identified a Plasmodium orthologue of Coq10 (PfCoq10), which is predominantly expressed in trophozoite-stage parasites, and localizes to the parasite mitochondrion. Expression of PfCoq10 in the S. cerevisiae coq10 deletion strain restored the capability of the yeast to grow on respiratory substrates, suggesting a remarkable functional conservation of this protein over a vast evolutionary distance, and despite a relatively low level of amino acid sequence identity. As the antimalarial drug atovaquone acts as a competitive inhibitor of CoQ, we assessed whether over-expression of PfCoq10 altered the atovaquone sensitivity in parasites and in yeast mitochondria, but found no alteration of its activity.
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Details
- Title
- Characterization of a Plasmodium falciparum Orthologue of the Yeast Ubiquinone-Binding Protein, Coq10p
- Creators
- Bethany J Jenkins - Drexel UniversityThomas M Daly - Drexel UniversityJoanne M Morrisey - Drexel UniversityMichael W Mather - Drexel UniversityAkhil B Vaidya - Drexel UniversityLawrence W Bergman - Drexel University
- Publication Details
- PloS one, v 11(3), pp e0152197-e0152197
- Publisher
- Public LIbrary of Science (PLOS)
- Grant note
- R01 AI098413 / NIAID NIH HHS R01 AI068137 / NIAID NIH HHS R56 AI028398 / NIAID NIH HHS R01 AI028398 / NIAID NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000372708900046
- Scopus ID
- 2-s2.0-85020332600
- Other Identifier
- 991019167440404721
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- Web of Science research areas
- Biochemistry & Molecular Biology