Journal article
Characterization of a bone marrow progenitor cell line to be used as a model for the study of susceptibility to HIV infection
Journal of neurovirology, Vol.12, pp.41-41
01 May 2006
Abstract
Human immunodeficiency virus type 1 (HIV-1) infection of the monocytic cell lineage is involved in the pathologic events associated with acquired immunodeficiency syndrome (AIDS) and HIV-1 dementia (HIVD). Interestingly, CD34+/CD38? progenitor cells within the bone marrow are refractile to HIV-1 infection, possibly due to their low level expression of HIV-1 co-receptors, CXCR4 and CCR5, which upon differentiation are up-regulated, increasing susceptibility to infection. Based on a number of considerations, the CD34+/CD38+ TF-1 erythromyeloid progenitor cell line was selected as a model to study the differentiation process of hematopoietic progenitor cells. Treatment of TF-1 cells with PMA results in the development of a macrophage-like morphology. In addition, the cell surface molecules CD34 and CD38 were down-regulated, while CD69 expression increased dramatically, indicating differentiation and activation, respectively. The HIV-1 co-receptor expression was considerably increased while CD4 was down-regulated. Following treatment with PMA, TF-1 cells secrete various cytokines that are able to stimulate undifferentiated TF-1 cells in the absence of PMA; these include IL-8, MIP-1alpha, MIP-1beta, and RANTES. TF-1 cells that were treated with conditioned media from PMA-treated cells exhibited a phenotype similar to that of PMA-treated cells with the exception of a lower level of CD4 down-regulation. The concurrent expression of the HIV-1 receptor and co-receptors may provide a window of opportunity for HIV-1 infection of this progenitor cell population. In summary, these studies suggest that the TF-1 cell line could serve as a model to study the susceptibility of bone marrow progenitor cells to HIV-1 infection.
Metrics
2 Record Views
Details
- Title
- Characterization of a bone marrow progenitor cell line to be used as a model for the study of susceptibility to HIV infection
- Creators
- F KrebsA AlexakiB IrishM NonnemacherE KilareskiB Wigdahl
- Publication Details
- Journal of neurovirology, Vol.12, pp.41-41
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Identifiers
- 991019170318504721