Journal article
Characterization of a mixed MRSA/MRSE biofilm in an explanted total ankle arthroplasty
FEMS immunology and medical microbiology, v 62(1), pp 66-74
01 Jun 2011
PMID: 21332826
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Abstract
Bacterial biofilms have been observed in many prosthesis-related infections, and this mode of growth renders the infection both difficult to treat and especially difficult to detect and diagnose using standard culture methods. We (1) tested a novel coupled PCR-mass spectrometric (PCR-MS) assay (the Ibis T5000) on an ankle arthroplasty that was culture negative on preoperative aspiration and then (2) confirmed that the Ibis assay had in fact detected a viable multispecies biofilm by further micrographic and molecular examinations, including confocal microscopy using Live/Dead stain, bacterial FISH, and reverse-transcriptase-PCR (RT-PCR) assay for bacterial mRNA. The Ibis technology detected Staphylococcus aureus, Staphylococcus epidermidis, and the methicillin resistance gene mecA in soft tissues associated with the explanted hardware. Viable S. aureus were confirmed using RT-PCR, and viable cocci in the biofilm configuration were detected microscopically on both tissue and hardware. Species-specific bacterial FISH confirmed a polymicrobial biofilm containing S. aureus. A novel culture method recovered S. aureus and S. epidermidis (both methicillin resistant) from the tibial metal component. These observations suggest that molecular methods, particularly the new Ibis methodology, may be a useful adjunct to routine cultures in the detection of biofilm bacteria in prosthetic joint infection.
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Details
- Title
- Characterization of a mixed MRSA/MRSE biofilm in an explanted total ankle arthroplasty
- Creators
- Paul Stoodley - Center for Genomic Sciences, Allegheny-Singer Research Institute, Allegheny General Hospital, Pittsburgh, PA, USAStephen F Conti - Department of Orthopedic Surgery, Allegheny General Hospital, Pittsburgh, PA, USAPatrick J DeMeo - Department of Orthopedic Surgery, Allegheny General Hospital, Pittsburgh, PA, USALaura Nistico - Center for Genomic Sciences, Allegheny-Singer Research Institute, Allegheny General Hospital, Pittsburgh, PA, USARachael Melton-Kreft - Center for Genomic Sciences, Allegheny-Singer Research Institute, Allegheny General Hospital, Pittsburgh, PA, USASandra Johnson - Center for Genomic Sciences, Allegheny-Singer Research Institute, Allegheny General Hospital, Pittsburgh, PA, USAAli Darabi - Department of Pathology and Laboratory Medicine, Allegheny General Hospital, Pittsburgh, PA, USAGarth D Ehrlich - Center for Genomic Sciences, Allegheny-Singer Research Institute, Allegheny General Hospital, Pittsburgh, PA, USAJ. William Costerton - Center for Genomic Sciences, Allegheny-Singer Research Institute, Allegheny General Hospital, Pittsburgh, PA, USASandeep Kathju - Center for Genomic Sciences, Allegheny-Singer Research Institute, Allegheny General Hospital, Pittsburgh, PA, USA
- Publication Details
- FEMS immunology and medical microbiology, v 62(1), pp 66-74
- Publisher
- Blackwell Publishing Ltd; Oxford, UK
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000289832700008
- Scopus ID
- 2-s2.0-79955036345
- Other Identifier
- 991014877944104721
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InCites Highlights
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Immunology
- Infectious Diseases
- Microbiology