Journal article
Characterization of bacterial communities in venous insufficiency wounds by use of conventional culture and molecular diagnostic methods
Journal of clinical microbiology, v 49(11), pp 3812-3819
Nov 2011
PMID: 21880958
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Microbial infections delay wound healing, but the effect of the composition of the wound microbiome on healing parameters is unknown. To better understand bacterial communities in chronic wounds, we analyzed debridement samples from lower-extremity venous insufficiency ulcers using the following: conventional anaerobic and aerobic bacterial cultures; the Ibis T5000 universal biosensor (Abbott Molecular); and 16S 454 FLX titanium series pyrosequencing (Roche). Wound debridement samples were obtained from 10 patients monitored clinically for at least 6 months, at which point 5 of the 10 sampled wounds had healed. Pyrosequencing data revealed significantly higher bacterial abundance and diversity in wounds that had not healed at 6 months. Additionally, Actinomycetales was increased in wounds that had not healed, and Pseudomonadaceae was increased in wounds that had healed by the 6-month follow-up. Baseline wound surface area, duration, or analysis by Ibis or conventional culture did not reveal significant differences between wounds that healed after 6 months and those that did not. Thus, pyrosequencing identified distinctive baseline characteristics of wounds that did not heal by the 6-month follow-up, furthering our understanding of potentially unique microbiome characteristics of chronic wounds.
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Details
- Title
- Characterization of bacterial communities in venous insufficiency wounds by use of conventional culture and molecular diagnostic methods
- Creators
- Marie S Tuttle - Case Western Reserve University, University Hospitals Case Medical Center, Department of Dermatology, Cleveland, OH 44106, USA. marie.tuttle@uhhospitals.orgEliot MostowPranab MukherjeeFen Z HuRachael Melton-KreftGarth D EhrlichScot E DowdMahmoud A Ghannoum
- Publication Details
- Journal of clinical microbiology, v 49(11), pp 3812-3819
- Publisher
- American Society for Microbiology (ASM); United States
- Grant note
- U19AI084024 / NIAID NIH HHS 1R21AI074077-01A2 / NIAID NIH HHS R01 AI080935 / NIAID NIH HHS R01 DE017486 / NIDCR NIH HHS R01 DC02148 / NIDCD NIH HHS R01 AI080935-01 / NIAID NIH HHS R01DE 13932-4 / NIDCR NIH HHS R01 AI035097 / NIAID NIH HHS R01 DE017486-01A1 / NIDCR NIH HHS R21 AI074077 / NIAID NIH HHS R01 DC002148 / NIDCD NIH HHS R01 DE013932 / NIDCR NIH HHS ACUREQ0600136 / PHS HHS U19 AI084024 / NIAID NIH HHS U01 DK082316 / NIDDK NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000296617300016
- Scopus ID
- 2-s2.0-80355141555
- Other Identifier
- 991014877805104721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Microbiology