Journal article
Characterization of glucose transport by bovine retinal capillary pericytes in culture
Experimental eye research, v 41(2), pp 191-199
1985
PMID: 3905422
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Abstract
The transport of
d-glucose and its non-metabolizable analog, 3-
o-methyl-
d-glucose (3-
o-MG), by cultured bovine retinal capillary pericytes was characterized. A preference for
d-glucose over
l-glucose uptake by pericytes indicated a stereospecific process. The 3-
o-MG transport appeated to follow complex kinetics. At low substrate concentrations, 3-
o-MG transport had an apparent
K
m
of 1·53 m
m and a
V
max
of 0·50 nmol (μg DNA)
−1 min
−1 at 37°C. A reduced extracellular sodium concentration, energy poisons, and exogenous insulin did not significantly influence 3-
o-MG uptake. Inhibition of the uptake or efflux of 3-
o-MG by cytochalasin B or phloretin, respectively, and the demonstration of a ‘counter transport’ phenomenon showed that 3-
o-MG transport by retinal capillary pericytes in culture was a carrier-mediated process.
Metrics
Details
- Title
- Characterization of glucose transport by bovine retinal capillary pericytes in culture
- Creators
- Weiye Li - Penn Presbyterian Medical CenterLawrence S. Chan - Penn Presbyterian Medical CenterMahin Khatami - Penn Presbyterian Medical CenterJohn H. Rockey - Penn Presbyterian Medical Center
- Publication Details
- Experimental eye research, v 41(2), pp 191-199
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Ophthalmology [Historical]
- Web of Science ID
- WOS:A1985ASE6700007
- Scopus ID
- 2-s2.0-0022377937
- Other Identifier
- 991019299114604721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Ophthalmology