Journal article
Characterization of integrated hepatitis B virus DNA harboring pre-S mutations in hepatocellular carcinoma patients with ground glass hepatocytes
Journal of medical virology, v 96(1), pe29348
Jan 2024
PMID: 38180275
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Ground glass hepatocytes (GGHs) have been associated with hepatocellular carcinoma (HCC) recurrence and poor prognosis. We previously demonstrated that pre-S expression in some GGHs is resistant to current hepatitis B virus (HBV) antiviral therapies. This study aimed to investigate whether integrated HBV DNA (iDNA) is the primary HBV DNA species responsible for sustained pre-S expression in GGH after effective antiviral therapy. We characterized 10 sets of micro-dissected, formalin-fixed-paraffin-embedded, and frozen GGH, HCC, and adjacent hepatitis B surface antigen-negative stained tissues for iDNA, pre-S deletions, and the quantity of covalently closed circular DNA. Eight patients had detectable pre-S deletions, and nine had detectable iDNA. Interestingly, eight patients had integrations within the TERT and CCNE1 genes, which are known recurrent integration sites associated with HCC. Furthermore, we observed a recurrent integration in the ABCC13 gene. Additionally, we identified variations in the type and quantity of pre-S deletions within individual sets of tissues by junction-specific PacBio long-read sequencing. The data from long-read sequencing indicate that some pre-S deletions were acquired following the integration events. Our findings demonstrate that iDNA exists in GGH and can be responsible for sustained pre-S expression in GGH after effective antiviral therapy.
Metrics
22 Record Views
Details
- Title
- Characterization of integrated hepatitis B virus DNA harboring pre-S mutations in hepatocellular carcinoma patients with ground glass hepatocytes
- Creators
- Yih-Ping Su - Department of Microbiology and Immunology, Drexel University College of Medicine, Philadelphia, Pennsylvania, USASelena Y Lin - JBS Science (United States)Ih-Jen Su - Department of BiotechnologyYu-Lan Kao - Baruch S. Blumberg InstituteShih-Chun Shen - Baruch S. Blumberg InstituteJoshua P Earl - Drexel UniversityGarth D Ehrlich - Drexel UniversityCheng-Yi Chen - National Cheng Kung UniversityWenya Huang - National Cheng Kung UniversityYing-Hsiu Su - Baruch S. Blumberg InstituteHung-Wen Tsai - National Cheng Kung University Hospital
- Publication Details
- Journal of medical virology, v 96(1), pe29348
- Publisher
- Wiley
- Grant note
- NCI NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:001158783800044
- Scopus ID
- 2-s2.0-85181696191
- Other Identifier
- 991021814915704721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Virology