Journal article
Characterization of mechanical behavior of an epithelial monolayer in response to epidermal growth factor stimulation
Experimental cell research, v 318(5), pp 521-526
10 Mar 2012
PMID: 22227009
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Cell signaling often causes changes in cellular mechanical properties. Knowledge of such changes can ultimately lead to insight into the complex network of cell signaling. In the current study, we employed a combination of atomic force microscopy (AFM) and quartz crystal microbalance with dissipation monitoring (QCM-D) to characterize the mechanical behavior of A431 cells in response to epidermal growth factor receptor (EGFR) signaling. From AFM, which probes the upper portion of an individual cell in a monolayer of cells, we observed increases in energy dissipation, Young's modulus, and hysteresivity. Increases in hysteresivity imply a shift toward a more fluid-like mechanical ordering state in the bodies of the cells. From QCM-D, which probes the basal area of the monolayer of cells collectively, we observed decreases in energy dissipation factor. This result suggests a shift toward a more solid-like state in the basal areas of the cells. The comparative analysis of these results indicates a regionally specific mechanical behavior of the cell in response to EGFR signaling and suggests a correlation between the time-dependent mechanical responses and the dynamic process of EGFR signaling. This study also demonstrates that a combination of AFM and QCM-D is able to provide a more complete and refined mechanical profile of the cells during cell signaling.
► The EGF-induced cellular mechanical response is regionally specific. ► The EGF-induced cellular mechanical response is time and dose dependent. ► A combination of AFM and QCM-D provides a more complete mechanical profile of cells.
Metrics
Details
- Title
- Characterization of mechanical behavior of an epithelial monolayer in response to epidermal growth factor stimulation
- Creators
- Ruiguo Yang - Department of Electrical and Computer Engineering, Michigan State University, East Lansing, Michigan 48824, United StatesJennifer Y Chen - Department of Chemistry, Drexel University, 3141 Chestnut Street, Philadelphia, Pennsylvania 19104, United StatesNing Xi - Department of Electrical and Computer Engineering, Michigan State University, East Lansing, Michigan 48824, United StatesKing Wai Chiu Lai - Department of Electrical and Computer Engineering, Michigan State University, East Lansing, Michigan 48824, United StatesChengeng Qu - Department of Electrical and Computer Engineering, Michigan State University, East Lansing, Michigan 48824, United StatesCarmen Kar Man Fung - Department of Electrical and Computer Engineering, Michigan State University, East Lansing, Michigan 48824, United StatesLynn S Penn - Department of Chemistry, Drexel University, 3141 Chestnut Street, Philadelphia, Pennsylvania 19104, United StatesJun Xi - Department of Chemistry, Drexel University, 3141 Chestnut Street, Philadelphia, Pennsylvania 19104, United States
- Publication Details
- Experimental cell research, v 318(5), pp 521-526
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Chemistry
- Web of Science ID
- WOS:000300966300010
- Scopus ID
- 2-s2.0-84862817425
- Other Identifier
- 991014877698104721
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InCites Highlights
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Cell Biology
- Oncology