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Journal article
Characterization of nontypable Haemophilus influenzae isolates recovered from adult patients with underlying chronic lung disease reveals genotypic and phenotypic traits associated with persistent infection
PloS one, v 9(5), pp e97020-e97020
2014
PMID: 24824990
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Nontypable Haemophilus influenzae (NTHi) has emerged as an important opportunistic pathogen causing infection in adults suffering obstructive lung diseases. Existing evidence associates chronic infection by NTHi to the progression of the chronic respiratory disease, but specific features of NTHi associated with persistence have not been comprehensively addressed. To provide clues about adaptive strategies adopted by NTHi during persistent infection, we compared sequential persistent isolates with newly acquired isolates in sputa from six patients with chronic obstructive lung disease. Pulse field gel electrophoresis (PFGE) identified three patients with consecutive persistent strains and three with new strains. Phenotypic characterisation included infection of respiratory epithelial cells, bacterial self-aggregation, biofilm formation and resistance to antimicrobial peptides (AMP). Persistent isolates differed from new strains in showing low epithelial adhesion and inability to form biofilms when grown under continuous-flow culture conditions in microfermenters. Self-aggregation clustered the strains by patient, not by persistence. Increasing resistance to AMPs was observed for each series of persistent isolates; this was not associated with lipooligosaccharide decoration with phosphorylcholine or with lipid A acylation. Variation was further analyzed for the series of three persistent isolates recovered from patient 1. These isolates displayed comparable growth rate, natural transformation frequency and murine pulmonary infection. Genome sequencing of these three isolates revealed sequential acquisition of single-nucleotide variants in the AMP permease sapC, the heme acquisition systems hgpB, hgpC, hup and hxuC, the 3-deoxy-D-manno-octulosonic acid kinase kdkA, the long-chain fatty acid transporter ompP1, and the phosphoribosylamine glycine ligase purD. Collectively, we frame a range of pathogenic traits and a repertoire of genetic variants in the context of persistent infection by NTHi.
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Details
- Title
- Characterization of nontypable Haemophilus influenzae isolates recovered from adult patients with underlying chronic lung disease reveals genotypic and phenotypic traits associated with persistent infection
- Creators
- Junkal Garmendia - Spanish National Research CouncilCristina Viadas - Universidad Publica de NavarraLaura Calatayud - Centro de Investigación Biomédica en Red de Enfermedades RespiratoriasJoshua Chang Mell - Drexel UniversityPau Martí-Lliteras - Centro de Investigación Biomédica en Red de Enfermedades RespiratoriasBegoña Euba - Instituto de Agrobiotecnología, CSIC-Universidad Pública Navarra-Gobierno Navarra, Mutilva, Spain Centro de Investigación Biomédica en Red de Enfermedades Respiratorias (CIBERES), Madrid, SpainEnrique Llobet - Centro de Investigación Biomédica en Red de Enfermedades RespiratoriasCarmen Gil - Universidad Publica de NavarraJosé Antonio Bengoechea - Spanish National Research CouncilRosemary J Redfield - University of British ColumbiaJosefina Liñares - Centro de Investigación Biomédica en Red de Enfermedades Respiratorias
- Publication Details
- PloS one, v 9(5), pp e97020-e97020
- Publisher
- Public LIbrary of Science (PLOS)
- Grant note
- 5F32AI084427 / NIAID NIH HHS F32 AI084427 / NIAID NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000336369200051
- Scopus ID
- 2-s2.0-84901261451
- Other Identifier
- 991019168119004721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Microbiology