Journal article
Characterization of norepinephrine accumulation by a crude synaptosomal-mitochondrial fraction isolated from rat heart
Life sciences (1973), v 48(13), pp 1317-1324
1991
PMID: 1848347
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Norepinephrine (NE) uptake into a heart synaptosomal-mitochondrial fraction was assessed under conditions where neuronal uptake (type 1) was linear with respect to both time and protein concentration. The NE accumulation process was sensitive to incubation temperature, sodium ion concentration and medium osmolality. Furthermore, NE uptake was attenuated by the neuronal uptake inhibitor desmethylimipramine (DMI) in a concentration dependent manner; the IC50 value was approximately 10 nM and maximum inhibition was obtained at 100 nM. In contrast, the extraneuronal uptake inhibitor, metanephrine did not significantly attenuate NE uptake. Kinetic analysis demonstrated that the DMI sensitive NE accumulation is saturable with a KM of approximately 400 nM and that NE uptake occurs via a single uptake process. This demonstration of neuronal type NE uptake by a synaptosomal-mitochondrial fraction constitutes a successful demonstration of the preparation of a rat heart subcellular fraction containing functional synaptosomes.
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Details
- Title
- Characterization of norepinephrine accumulation by a crude synaptosomal-mitochondrial fraction isolated from rat heart
- Creators
- V J Aloyo - Drexel UniversityH B McIlvain - Drexel UniversityV H Bhavsar - Drexel UniversityJ Roberts - Drexel University
- Publication Details
- Life sciences (1973), v 48(13), pp 1317-1324
- Publisher
- Elsevier
- Grant note
- AG03362 / NIA NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pharmacology and Physiology; Emergency Medicine
- Web of Science ID
- WOS:A1991EZ25100012
- Scopus ID
- 2-s2.0-0026092476
- Other Identifier
- 991019184102504721
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- Collaboration types
- International collaboration
- Web of Science research areas
- Medicine, Research & Experimental
- Pharmacology & Pharmacy