Journal article
Characterization of striatal neurons expressing high levels of glutamic acid decar☐ylase messenger RNA
Brain research, v 492(1)
1989
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Two types of labelled cells are detected in sections of rat and mouse striata processed for in situ hybridization histochemistry with
35S-radiolabelled RNA probes complementary to the messenger RNA (mRNA) encoding glutamic acid decar☐ylase (GAD), the synthesis enzyme for γ-aminobutyric acid (GABA): numerous lightly, and fewer very densely labelled neurons. In order to determine whether the densely labelled cells correspond to the striatal somatostatinergic neurons with which they share morphological characteristics, the presence of GAD mRNA was examined in brain sections processed successively for dihydronicotinamide adenine dinucleotide phosphate (NADPH) diaphorase histochemistry, a marker of striatal somatostatinergic neurons, and in situ hybridization histochemistry. In addition, the distribution of GABAergic interneurons was analysed with regard to striatal compartments (striosomes) indicated by patches of dense opiate binding sites. The results show that NADPH diaphorase activity and GAD mRNA do not co-exist in striatal neurons. Furthermore, in contrast to the somatostatinergic neurons which are almost exclusively located in the extrastriosomal matrix, densely labelled GAD cells were present both in the striosomes and the matrix, further suggesting that GABAergic and somatostatinergic neurons form two distinct interneuronal systems in the striatum of rats and mice.
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Details
- Title
- Characterization of striatal neurons expressing high levels of glutamic acid decar☐ylase messenger RNA
- Creators
- Marie-Francoise Chesselet - Drexel UniversityElaine Robbins - Drexel UniversityEli Wr Robbins - Interior Design
- Publication Details
- Brain research, v 492(1)
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Interior Design
- Web of Science ID
- WOS:A1989AF63900024
- Scopus ID
- 2-s2.0-0024392812
- Other Identifier
- 991019184176204721
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- Web of Science research areas
- Neurosciences