Journal article
Characterization of the ion channel activity in planar bilayers containing IgE-Fc sub( epsilon ) receptor and the cromolyn-binding protein
The EMBO journal, Vol.5(5), pp.849-854
01 Jan 1986
Abstract
Electric conductance was studied across micropipette-supported planar lipid bilayers, reconstituted with IgE-Fc sub( epsilon ) receptor and the cromolyn-binding protein (CBP) isolated from membranes of rat basophilic leukemia cells (RBL-2H3). Currents were observed following the addition of aggregating agents, specific for either of the two proteins. The results show that the two proteins are necessary and sufficient for the opening of cation channels. Channels induced by antigen were susceptible to blocking by the anti-allergic drug cromolyn. The results suggest that CBP acts as the core of the cation channel and that the channel conductance and open-time characteristics are independent of the mode of aggregation.
Metrics
8 Record Views
Details
- Title
- Characterization of the ion channel activity in planar bilayers containing IgE-Fc sub( epsilon ) receptor and the cromolyn-binding protein
- Creators
- A CorciaR Schweitzer-StennerI PechtB Rivnay
- Publication Details
- The EMBO journal, Vol.5(5), pp.849-854
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- [Retired Faculty]
- Identifiers
- 991019196793004721