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Journal article
Characterization of the size and 5′ cap of messenger RNA encoding neurophysin precursors
Nucleic acids research, v 12(10), pp 4397-4410
25 May 1984
PMID: 6145145
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The coding activity of bovine hypothalamic poly A+ mRNA for neurophysin I and II immunoreactive proteins was charscterized with respect to size and 5′ cap. The mRNA was fractionated by methylmercuric hydroxide agarose gel electrophoresis and subsequently translated in vitro in rabbit reticulocyte lysates. Alternatively, nRNA was fractionated by gel exclusion HPLC and translated in wheat germ extracts. Imnunoprecipitated translation products were analyzed by gel exclusion HPLC. Neurophysin-immunoapecific protein of ∼17,000 daltons, the size expected for the neuropeptide hormone-neurophysin precursors, was encoded by mRNA species of two size classes. The smaller class of mRNA's was of the size expected from the size of the precursor proteins. The larger class was 5–10 times larger. The low K+ concentration optimum for translation of unfractionated mRNA encoding neurophysin I-immunoreactive proteins and the inability of a cap analogue to inhibit this translation suggest that mRNA species encoding neurophysin I-immunoreactive translation products are incompletely capped. By contrast, the mRNA encoding neurophysin II-immunoreactive products appear to contain a normal cap structure.
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Details
- Title
- Characterization of the size and 5′ cap of messenger RNA encoding neurophysin precursors
- Creators
- Christopher J. Hough - National Institute of Diabetes and Digestive and Kidney DiseasesIrwin M. Chaiken - National Institute of Diabetes and Digestive and Kidney Diseases
- Publication Details
- Nucleic acids research, v 12(10), pp 4397-4410
- Publisher
- Oxford University Press
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology; Drexel University
- Web of Science ID
- WOS:A1984ST89700026
- Scopus ID
- 2-s2.0-0021770547
- Other Identifier
- 991019520530104721
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- Web of Science research areas
- Biochemistry & Molecular Biology