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Characterization of two plasma membrane proteins abundant in rat brain
Journal article   Peer reviewed

Characterization of two plasma membrane proteins abundant in rat brain

Hans Werner Steisslinger, Vincent J. Aloyo and Ljubisˇa Vitković
Brain research, v 415(2), pp 375-379
1987
PMID: 3607506

Abstract

Abundant protein Phosphorylation Plasma membrane Rat brain cortex Two-dimensional electrophoresis
Plasma membranes were isolated from rat brain cortices and their proteins characterized by two-dimensional electrophoresis. Approximately 500 polypeptides with relative molecular weights (mol. wt.) between 20 kDa and 120 kDa and isoelectric points (pI) between 4.2 and 8.5 were visualized by silver staining. Two proteins, MP 1 and MP 2, comprised about 5% each of the total by mass. Their mol. wts. were 56 kDa and 43 kDa, and their pIs were 4.2 and 4.3, respectively. The two proteins were present in membranes of cultured granule neurons and cortical astrocytes and absent in liver and kidney. They were both substrates for phosphorylation by protein kinase C. MP 2 is similar, if not identical, to a major phosphoprotein in growth cones, pp46 (also termed GAP-43, B-50, F1).

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Collaboration types
Domestic collaboration
Web of Science research areas
Neurosciences
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