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Accepted (AM)Open Access (License Unspecified), Open
Journal article
Charge-Neutral, Stable, Non-Cytotoxic, Near-Infrared SnS Aqueous Quantum Dots for High Signal-to-Noise-Ratio Biomedical Imaging
ChemistrySelect (Weinheim), v 2(24), pp 7332-7339
22 Aug 2017
PMID: 30410961
Abstract
We have synthesized charge-neutral, stable, non-cytotoxic, bright, near-infrared (NIR) SnS quantum dots (QDs) by first making Cysteamine-capped SnS QDs in glycerol under acidic conditions followed by lengthening the capping molecule with peptide bonds by reacting the capping molecules with glycine and subsequent heat treatment at 200 degrees C for 4 hours. The obtained stable SnS QDs exhibited a band gap of 1.5 eV and a strong, narrow NIR emission peak at 830 nm with a quantum yield of 4.6 %. The suspension could be stable for more than 1 month without aggregation or emission decay. The positively charged SnS QDs were further neutralized by 3-mercaptoproprionic acid (MPA) through electrostatic attraction. The MPA neutralized SnS QDs were shown to be non-cytotoxic at concentrations 6 times the typical QDs concentration for immunostaining. Low-noise, optimal NIR immunofluorescent imaging of vascular endothelial growth factor (VEGF) on 3T3 cells and Tn antigen on HT29 cells was achieved by using streptavidin (SA)-linked MPA-neutralized SnS QDs with a SA: QD molar ratio of 22:1 to bind to biotinylated secondary antibody bound on the primary antibody that was bound on the targeted antigen on the cell membrane with a high signal-to noise ratio (SNR) of 35.
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Details
- Title
- Charge-Neutral, Stable, Non-Cytotoxic, Near-Infrared SnS Aqueous Quantum Dots for High Signal-to-Noise-Ratio Biomedical Imaging
- Creators
- Song Han - Drexel UniversityWan Y. Shih - Drexel UniversityWei-Heng Shih - Drexel University
- Publication Details
- ChemistrySelect (Weinheim), v 2(24), pp 7332-7339
- Publisher
- Wiley
- Number of pages
- 8
- Grant note
- 1R41AI112224; 1R41AI120445 / National Institute of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA R41AI120445 / NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Allergy & Infectious Diseases (NIAID) Coulter-Drexel Translational Research Partnership grant
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems; Materials Science and Engineering
- Web of Science ID
- WOS:000408644100056
- Scopus ID
- 2-s2.0-85041799502
- Other Identifier
- 991019167634204721
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Chemistry, Multidisciplinary