Journal article
Chemical Heating for Minimally Instrumented Point-of-Care (POC) Molecular Diagnostics
Biosensors (Basel), v 14(11), p554
13 Nov 2024
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The minimal instrumentation of portable medical diagnostic devices for point-of-care applications is facilitated by using chemical heating in place of temperature-regulated electrical heaters. The main applications are for isothermal nucleic acid amplification tests (NAATs) and other enzymatic assays that require elevated, controlled temperatures. In the most common implementation, heat is generated by the exothermic reaction of a metal (e.g., magnesium, calcium, or lithium) with water or air, buffered by a phase-change material that maintains a near-constant temperature to heat the assay reactions. The ability to incubate NAATs electricity-free and to further to detect amplification with minimal instrumentation opens the door for fully disposable, inexpensive molecular diagnostic devices that can be used for pathogen detection as needed in resource-limited areas and during natural disasters, wars, and civil disturbances when access to electricity may be interrupted. Several design approaches are reviewed, including more elaborate schemes for multiple stages of incubation at different temperatures.
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Details
- Title
- Chemical Heating for Minimally Instrumented Point-of-Care (POC) Molecular Diagnostics
- Creators
- Michael G. Mauk - University of PennsylvaniaFelix Ansah - University of GhanaMohamed El-Tholoth - Mansoura University
- Publication Details
- Biosensors (Basel), v 14(11), p554
- Publisher
- MDPI; BASEL
- Number of pages
- 19
- Grant note
- Higher Colleges of Technology, Interdisciplinary research: grant-212335 Higher Colleges of TechnologyScience for Africa Foundation: DEL-22-014 Science in Africa (DELTAS Africa) ProgrammeWellcome TrustCommonwealth & Development Office
This work was supported by a grant awarded by the Higher Colleges of Technology, Interdisciplinary research grant-212335, United Arab Emirates. F.A. was partly supported by a grant from the Science for Africa Foundation to the Developing Excellence in Leadership, Training and Science in Africa (DELTAS Africa) Programme [DEL-22-014], with support from Wellcome Trust and the UK Foreign, Commonwealth & Development Office.
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Engineering Leadership and Society/Engineering Technology
- Web of Science ID
- WOS:001363964900001
- Scopus ID
- 2-s2.0-85210183236
- Other Identifier
- 991021962014804721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Chemistry, Analytical
- Instruments & Instrumentation
- Nanoscience & Nanotechnology