Journal article
Chemokine-adjuvanted electroporated DNA vaccine induces substantial protection from simian immunodeficiency virus vaginal challenge
Mucosal immunology, v 9(1), pp 13-23
Jan 2016
PMID: 25943275
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
There have been encouraging results for the development of an effective HIV vaccine. However, many questions remain regarding the quality of immune responses and the role of mucosal antibodies. We addressed some of these issues by using a simian immunodeficiency virus (SIV) DNA vaccine adjuvanted with plasmid-expressed mucosal chemokines combined with an intravaginal SIV challenge in rhesus macaque (RhM) model. We previously reported on the ability of CCR9 and CCR10 ligand (L) adjuvants to enhance mucosal and systemic IgA and IgG responses in small animals. In this study, RhMs were intramuscularly immunized five times with either DNA or DNA plus chemokine adjuvant delivered by electroporation followed by challenge with SIVsmE660. Sixty-eight percent of all vaccinated animals (P<0.01) remained either uninfected or had aborted infection compared with only 14% in the vaccine naïve group. The highest protection was observed in the CCR10L chemokines group, where six of nine animals had aborted infection and two remained uninfected, leading to 89% protection (P<0.001). The induction of mucosal SIV-specific antibodies and neutralization titers correlated with trends in protection. These results indicate the need to further investigate the contribution of chemokine adjuvants to modulate immune responses and the role of mucosal antibodies in SIV/HIV protection.
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Details
- Title
- Chemokine-adjuvanted electroporated DNA vaccine induces substantial protection from simian immunodeficiency virus vaginal challenge
- Creators
- M A Kutzler - Division of Infectious Diseases and HIV Medicine, The Department of Medicine, Drexel University College of Medicine, Philadelphia, Pennsylvania, USAM C Wise - Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA, U.S.AN A Hutnick - Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA, U.S.AZ Moldoveanu - Department of Microbiology and Medicine, University of Alabama at Birmingham, Birmingham, Alabama, USAM Hunter - Tulane National Primate Research Center and the Department of Tropical Medicine, School of Public Health and Tropical Medicine, Tulane University, Covington, Louisiana, USAM Reuter - Department of Microbiology and Immunology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, USAS Yuan - Department of Microbiology and Immunology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, USAJ Yan - Inovio Pharmaceuticals, Plymouth Meeting, Pennsylvania, USAA Ginsberg - Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA, U.S.AA Sylvester - Inovio Pharmaceuticals, Plymouth Meeting, Pennsylvania, USAB Pahar - Tulane National Primate Research Center and the Department of Tropical Medicine, School of Public Health and Tropical Medicine, Tulane University, Covington, Louisiana, USAD Carnathan - Department of Microbiology and Immunology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, USAN Kathuria - Division of Infectious Diseases and HIV Medicine, The Department of Medicine, Drexel University College of Medicine, Philadelphia, Pennsylvania, USAA S Khan - Inovio Pharmaceuticals, Plymouth Meeting, Pennsylvania, USAD Montefiori - Human Vaccine Institute, Duke University, Durham, North Carolina, USAN Y Sardesai - Inovio Pharmaceuticals, Plymouth Meeting, Pennsylvania, USAM R Betts - Department of Microbiology and Immunology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, USAJ Mestecky - Department of Microbiology and Medicine, University of Alabama at Birmingham, Birmingham, Alabama, USAP Marx - Tulane National Primate Research Center and the Department of Tropical Medicine, School of Public Health and Tropical Medicine, Tulane University, Covington, Louisiana, USAD B Weiner - Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA, U.S.A
- Publication Details
- Mucosal immunology, v 9(1), pp 13-23
- Publisher
- Springer Nature; United States
- Grant note
- F32 AI054152 / NIAID NIH HHS P51 RR000164 / NCRR NIH HHS U19AI078675 / NIAID NIH HHS P30 AI045008 / NIAID NIH HHS P01 AI071739 / NIAID NIH HHS P01AI071739 / NIAID NIH HHS P51 RR00164/OD01104 / NIH HHS P51 OD011104 / NIH HHS U19 AI109646 / NIAID NIH HHS N01A1154 / PHS HHS F32AI054152 / NIAID NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Infectious Diseases (and HIV Medicine)
- Web of Science ID
- WOS:000367653800002
- Scopus ID
- 2-s2.0-84953320196
- Other Identifier
- 991014878136704721
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- Collaboration types
- Industry collaboration
- Domestic collaboration
- Web of Science research areas
- Immunology