Journal article
Chemoreceptor co-expression in Drosophila melanogaster olfactory neurons
eLife, v 11, e72599
20 Apr 2022
PMID: 35442190
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
olfactory neurons have long been thought to express only one chemosensory receptor gene family. There are two main olfactory receptor gene families in
, the odorant receptors (ORs) and the ionotropic receptors (IRs). The dozens of odorant-binding receptors in each family require at least one co-receptor gene in order to function:
for ORs, and
,
, and
for IRs. Using a new genetic knock-in strategy, we targeted the four co-receptors representing the main chemosensory families in
(
). Co-receptor knock-in expression patterns were verified as accurate representations of endogenous expression. We find extensive overlap in expression among the different co-receptors. As defined by innervation into antennal lobe glomeruli,
is broadly expressed in 88% of all olfactory sensory neuron classes and is co-expressed in 82% of Orco+ neuron classes, including all neuron classes in the maxillary palp.
,
, and
expression patterns are also more expansive than previously assumed. Single sensillum recordings from Orco-expressing
mutant antennal and palpal neurons identify changes in olfactory responses. We also find co-expression of
and
in
and
olfactory neurons. These results suggest that co-expression of chemosensory receptors is common in insect olfactory neurons. Together, our data present the first comprehensive map of chemosensory co-receptor expression and reveal their unexpected widespread co-expression in the fly olfactory system.
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Details
- Title
- Chemoreceptor co-expression in Drosophila melanogaster olfactory neurons
- Creators
- Darya Task - Johns Hopkins MedicineChun-Chieh Lin - Johns Hopkins MedicineAlina Vulpe - University of ConnecticutAli Afify - Johns Hopkins MedicineSydney Ballou - University of ConnecticutMaria Brbic - Stanford UniversityPhilipp Schlegel - University of CambridgeJoshua Raji - Johns Hopkins MedicineGregory Jefferis - MRC Laboratory of Molecular BiologyHongjie Li - Howard Hughes Medical InstituteKaren Menuz - University of ConnecticutChristopher J Potter - Johns Hopkins Medicine
- Publication Details
- eLife, v 11, e72599
- Publisher
- eLife
- Grant note
- MC_U105188491 / Medical Research Council 203261/Z/16/Z / Wellcome Trust R21 DC017868 / NIDCD NIH HHS R01 AI137078 / NIAID NIH HHS R00 AG062746 / NIA NIH HHS R35 GM133209 / NIGMS NIH HHS P30 DC005211 / NIDCD NIH HHS RF1 MH120679 / NIMH NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biology
- Web of Science ID
- WOS:000807658500001
- Scopus ID
- 2-s2.0-85128799395
- Other Identifier
- 991021229986704721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biology