Journal article
Chiral cyclodextrin-modified microemulsion electrokinetic chromatography
Electrophoresis, v 25(9), pp 1188-1200
May 2004
PMID: 15174038
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Cyclodextrin (CD)-modified microemulsion electrokinetic chromatography (MEEKC) or CD-MEEKC has not previously been applied to the area of chiral separations. Herein, the results of investigations of various microemulsions with CD additives are presented. Two different microemulsions are explored: an ethyl acetate sodium dodecyl sulfate microemulsion, and a chiral dodecoxycarbonylvaline (DDCV) microemulsion. Each microemulsion is paired separately with a neutral CD (hydroxypropyl-beta-CD) and an anionic CD (sulfated-beta-CD). In addition, the chiral DDCV microemulsion is investigated in both the R- and S- form. By varying simple parameters such as buffer system, applied voltage, surfactant enantiomer, and type of cyclodextrin, dramatic improvements in the chiral separations were noted. Resolution was found to be highly dependent on buffer identity and concentration, and somewhat dependent on whether the CDs used were randomly or highly sulfated. Under optimized conditions, the resolution ranged from 0.8 to 4.8, with plate counts ranging from 4000 to 26 000. Additionally, S- and R-levetiracetam, which had never before been enantioseparated via capillary electrophoresis (CE) methodologies, were separated in less than 8 min, with a resolution of 1.1.
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Details
- Title
- Chiral cyclodextrin-modified microemulsion electrokinetic chromatography
- Creators
- Melissa D Mertzman - Department of Chemistry, Drexel University, Philadelphia, PA, USAJoe P Foley
- Publication Details
- Electrophoresis, v 25(9), pp 1188-1200
- Publisher
- Wiley; Germany
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Chemistry
- Web of Science ID
- WOS:000221735100003
- Scopus ID
- 2-s2.0-3042526365
- Other Identifier
- 991014877991204721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemical Research Methods
- Chemistry, Analytical