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Chlorine and ozone disinfection of Encephalitozoon intestinalis spores
Journal article   Peer reviewed

Chlorine and ozone disinfection of Encephalitozoon intestinalis spores

David E John, Charles N Haas, Nena Nwachuku and Charles P Gerba
Water research (Oxford), v 39(11), pp 2369-2375
2005
PMID: 15921720

Abstract

Disinfection Emerging pathogen Inactivation Microsporidia Waterborne parasites
Microsporidia are intracellular eukaryotic parasites which have the potential for zoonotic and environmental, including waterborne, transmission. Encephalitozoon intestinalis is a microsporidian pathogen of humans and animals and has been detected in surface water. It is also on the Contaminant Candidate List of potential emerging waterborne pathogens for the US EPA. We performed disinfection studies using chlorine and ozone on E. intestinalis spores with a cell-culture most-probable-number assay to determine infectivity. Chlorine experiments were performed at 5 °C at pH of 6, 7, and 8 with 1 mg/L initial chlorine concentrations, while ozone experiments were performed at 5 °C and pH 7 with initial ozone doses of 1 and 0.5 mg/L, both in buffered water. A derivation of Hom's model for disinfection kinetics under dynamic disinfectant concentrations was used to fit observed data and calculate concentration-time product ( C * t ) values. Chlorine C * t values varied with pH such that 99% (2-log 10) C * t ranged from 12.8 at pH 6 to 68.8 at pH 8 (mg min/L). Ozone C * t values were approximately an order of magnitude less at 0.59–0.84 mg min/L, depending on initial concentration.

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Collaboration types
Domestic collaboration
Web of Science research areas
Engineering, Environmental
Environmental Sciences
Water Resources
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