Journal article
Cholecystokinin A and B receptors are differentially expressed in normal pancreas and pancreatic adenocarcinoma
The Journal of clinical investigation, v 100(3), pp 597-603
01 Aug 1997
PMID: 9239407
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Cholecystokinin (CCK) plays an important role in pancreatic carcinogenesis. While human CCK-A and -B receptors have been fully characterized, their relative roles in human pancreatic adenocarcinoma remain unclear. Thus, expression of CCK-A and -B receptors in normal human pancreas, pancreatic adenocarcinomas, and other human extrapancreatic tissues and malignancies was examined, using reverse transcription followed by the polymerase chain reaction (RT-PCR). mRNA isolated from 15 normal pancreas specimens, 22 pancreatic adenocarcinomas, and 58 extrapancreatic tissues and tumors was subjected to RT-PCR using primers specific for human CCK-A and -B receptors. Expression of CCK-B receptors was detected in all tissues arising from pancreas and in most extrapancreatic tissues and tumors. In contrast, CCK-A receptors exhibited a more selective pattern of expression in gall bladder, intestine, brain, ovary, spleen, and thymus. Of significance, CCK-A receptors were expressed selectively in all pancreatic adenocarcinomas, but not in any normal pancreas specimens. In situ hybridization, using receptor-specific riboprobes, localized CCK-A receptor expression to ductal cells, the presumed origin of most human pancreatic adenocarcinomas. Southern blot analysis revealed no evidence of CCK-A receptor gene amplification or rearrangement in pancreatic adenocarcinomas. Because of its selective expression, the CCK-A receptor may serve as selective biomarker for pancreatic adenocarcinoma.
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Details
- Title
- Cholecystokinin A and B receptors are differentially expressed in normal pancreas and pancreatic adenocarcinoma
- Creators
- D S Weinberg - Thomas Jefferson UniversityB RuggeriM T BarberS BiswasS MiknyockiS A Waldman
- Publication Details
- The Journal of clinical investigation, v 100(3), pp 597-603
- Publisher
- American Society for Clinical Investigation
- Grant note
- CA 71080 / NCI NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pediatrics
- Web of Science ID
- WOS:A1997XQ28000015
- Scopus ID
- 2-s2.0-0030762567
- Other Identifier
- 991019167870004721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Medicine, Research & Experimental