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Journal article
Chondrocytes Contain a Growth Factor That is Localized in the Nucleus and is Associated with Chromatin
Proceedings of the National Academy of Sciences - PNAS, v 77(5), pp 2762-2766
01 May 1980
PMID: 6930665
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Abstract
Bovine scapular and articular chondrocytes were isolated from fresh cartilage and disrupted by sonication. The disrupted cells had the ability to stimulate DNA synthesis and cell division in vitro in chondrocytes and in 3T3 cells. Subcellular fractions were prepared by two methods, enucleation with cytochalasin B and lysis of cells with Nonidet P-40. After enucleation of chondrocytes, karyoplasts and cytoplasts were collected, disrupted by sonication, and tested for their ability to stimulate DNA synthesis. Over 95% of the cellular growth factor activity was localized in the karyoplast. In addition, after lysis of chondrocytes in Nonidet P-40, over 95% of the growth factor activity was recovered in the nuclear fraction. Chondrocyte chromatin was prepared by low ionic strength detergent treatment of karyoplasts. All of the growth factor activity of the karyoplast was found to be associated with chromatin. The growth factor activity of chondrocytes, cytoplasts, karyoplasts, and chromatin was analyzed by gel filtration on Bio-Gel A-0.5 m equilibrated with 4 M guanidine HCl and 5 mM dithiothreitol. Chondrocytes, chondrocyte karyoplasts, and chondrocyte chromatin had similar column elution profiles, with molecular weights in the range of 12,000-22,000.
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Details
- Title
- Chondrocytes Contain a Growth Factor That is Localized in the Nucleus and is Associated with Chromatin
- Creators
- Jane Clifford AzizkhanMichael Klagsbrun
- Publication Details
- Proceedings of the National Academy of Sciences - PNAS, v 77(5), pp 2762-2766
- Publisher
- National Academy of Sciences of the United States of America
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:A1980JU56800082
- Scopus ID
- 2-s2.0-0019221862
- Other Identifier
- 991020200886804721
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