Journal article
Chromatographic performance of large-pore versus small-pore columns in micellar liquid chromatography
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, v 785(1)
2003
PMID: 12535834
Abstract
Micellar liquid chromatography (MLC) is useful in bioanalysis because proteinaceous biofluids can be directly injected onto the column. The technique has been limited in part because of the apparently weak eluting power of micellar mobile phases. It has recently been shown [Anal. Chem. 72 (2000) 294] that this may be overcome by the use of large pore size stationary phases. In this work, large-pore (1000 Å) C
18 stationary phases were evaluated relative to conventional small-pore (100 Å) C
18 stationary phases for the direct sample injection of drugs in plasma. Furthermore, the difference between the large and small pore phases in gradient elution separations of mixtures of widely varying hydrophobicities was investigated. Large-pore stationary phases were found to be very effective for eluting moderately to highly hydrophobic compounds such as ibuprofen, crotamiton, propranolol, and dodecanophenone, which were highly retained on the small-pore stationary phases typically used in MLC. The advantages of direct introduction of biological samples (drugs in plasma) and rapid column re-equilibration after gradient elution in MLC were maintained with large-pore phases. Finally, recoveries, precision, linearity, and detection limits for the determination of quinidine and DPC 961 in spiked bovine plasma were somewhat better using MLC with wide pore phases.
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Details
- Title
- Chromatographic performance of large-pore versus small-pore columns in micellar liquid chromatography
- Creators
- Timothy J McCormick - Pharmaceutical Research and Development, DuPont Pharmaceuticals Company, Wilmington, DE 19880, USAJoe P Foley - Department of Chemistry, Villanova University, Villanova, PA 19085, USADavid K Lloyd - Pharmaceutical Research and Development, DuPont Pharmaceuticals Company, Wilmington, DE 19880, USA
- Publication Details
- Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, v 785(1)
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Chemistry
- Web of Science ID
- WOS:000180592700001
- Scopus ID
- 2-s2.0-0242516118
- Other Identifier
- 991014878527704721
InCites Highlights
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- Collaboration types
- Industry collaboration
- Domestic collaboration
- Web of Science research areas
- Biochemical Research Methods
- Chemistry, Analytical