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Journal article
Chronic neuronal activation increases dynamic microtubules to enhance functional axon regeneration after dorsal root crush injury
Nature communications, v 11(1), pp 6131-6131
30 Nov 2020
PMID: 33257677
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
After a dorsal root crush injury, centrally-projecting sensory axons fail to regenerate across the dorsal root entry zone (DREZ) to extend into the spinal cord. We find that chemogenetic activation of adult dorsal root ganglion (DRG) neurons improves axon growth on an in vitro model of the inhibitory environment after injury. Moreover, repeated bouts of daily chemogenetic activation of adult DRG neurons for 12 weeks post-crush in vivo enhances axon regeneration across a chondroitinase-digested DREZ into spinal gray matter, where the regenerating axons form functional synapses and mediate behavioral recovery in a sensorimotor task. Neuronal activation-mediated axon extension is dependent upon changes in the status of tubulin post-translational modifications indicative of highly dynamic microtubules (as opposed to stable microtubules) within the distal axon, illuminating a novel mechanism underlying stimulation-mediated axon growth. We have identified an effective combinatory strategy to promote functionally-relevant axon regeneration of adult neurons into the CNS after injury.
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Details
- Title
- Chronic neuronal activation increases dynamic microtubules to enhance functional axon regeneration after dorsal root crush injury
- Creators
- Di Wu - Drexel UniversityYing Jin - Drexel UniversityTatiana M. Shapiro - Drexel UniversityAbhishek Hinduja - Drexel UniversityPeter W. Baas - Drexel UniversityVeronica J. Tom - Drexel University
- Publication Details
- Nature communications, v 11(1), pp 6131-6131
- Publisher
- Springer Nature
- Number of pages
- 16
- Grant note
- 259350 / Craig H. Neilsen Foundation R01 NS085426; R01 NS106908; R01 NS111761; R01 NS28785; R01 NS118117 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA W81XWH1210379 / U.S. Army Medical Research and Materiel Command; U.S. Army Medical Research & Materiel Command (USAMRMC)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000617695100014
- Scopus ID
- 2-s2.0-85096938819
- Other Identifier
- 991019167603004721
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- Web of Science research areas
- Neurosciences