Journal article
Chronic subacromial impingement leads to supraspinatus muscle functional and morphological changes: Evaluation in a murine model
JOURNAL OF ORTHOPAEDIC RESEARCH, v 39(10), pp 2243-2251
Oct 2021
PMID: 33336819
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Muscle atrophy and fatty infiltration have been directly correlated with higher rates of incomplete or failed healing following surgical repair of the rotator cuff. The purpose of this study was to evaluate clinically relevant functional and morphological changes in the supraspinatus muscle at various time points in this model of rotator cuff tendinopathy. Subacromial impingement was induced in 47, male C57BL/6 mice (total 94 limbs) by implantation of a metal clip in the subacromial space. Specimens were evaluated at 4, 6, and 12 weeks postoperatively. Gait analysis was used to measure various kinematic parameters. Supraspinatus muscle wet weight, histology, and quantitative reverse-transcription polymerase chain reaction analysis of genes related to muscle atrophy and adipogenesis were performed to characterize the structural, cellular, and molecular changes. Muscle atrophy and fatty infiltration was evident beginning at 6 weeks, with progression out to 12 weeks. Gait analysis identified significant functional changes in many aspects of gait and abnormal stance tracing as early as 4 weeks, verifying alterations in upper extremity function. We have demonstrated that clinically relevant changes to the supraspinatus muscle are seen starting 6 weeks after induction of subacromial impingement. Furthermore, the gait analysis provides key functional outcome measurements that may be useful for future evaluation of new therapeutic strategies.
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Details
- Title
- Chronic subacromial impingement leads to supraspinatus muscle functional and morphological changes: Evaluation in a murine model
- Creators
- Brett J Croen - Drexel UniversityCamila B Carballo - Hospital for Special SurgerySusumu Wada - Hospital for Special SurgeryXueying Zhang - Hospital for Special SurgerySaral Patel - Hospital for Special SurgeryXiang-Hua Deng - Hospital for Special SurgeryScott A Rodeo - Hospital for Special Surgery
- Publication Details
- JOURNAL OF ORTHOPAEDIC RESEARCH, v 39(10), pp 2243-2251
- Publisher
- WILEY; HOBOKEN
- Number of pages
- 8
- Grant note
- Scott Rodeo has received funding from the National Institutes of Health, Orthopaedic Research and Education Foundation, Virginia Toulmin Foundation, and Angiocrine Biosciences.
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- College of Medicine
- Web of Science ID
- WOS:000603159500001
- Scopus ID
- 2-s2.0-85098119248
- Other Identifier
- 991021860659004721
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- Web of Science research areas
- Orthopedics