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Chronic surgical site infection due to suture-associated polymicrobial biofilm
Journal article   Open access   Peer reviewed

Chronic surgical site infection due to suture-associated polymicrobial biofilm

Sandeep Kathju, Laura Nistico, Luanne Hall-Stoodley, J Christopher Post, Garth D Ehrlich and Paul Stoodley
Surgical infections, v 10(5), pp 457-461
Oct 2009
PMID: 19811056
url
https://doi.org/10.1089/sur.2008.062View
Published, Version of Record (VoR) Open

Abstract

Corynebacterium - isolation & purification Humans Biofilms - growth & development In Situ Hybridization, Fluorescence Corynebacterium Infections - microbiology Microscopy, Confocal Staphylococcus - isolation & purification Adult Female Staphylococcal Infections - microbiology Sutures - microbiology Surgical Wound Infection - microbiology Chronic Disease
Surgical site infection (SSI) is a common surgical complication; culture-negative SSI presents a particular problem in management. Examination of explanted foreign bodies (sutures) using confocal laser scanning microscopy (CLSM) and fluorescent in situ hybridization (FISH) after surgical exploration of a chronic culture-negative SSI. Confocal microscopy (CM) demonstrated bacilli and cocci attached to the surface of the explanted sutures in a mixed biofilm. Fluorescent in situ hybridization confirmed that Staphylococci were components of the mixed biofilm. Removal of the foreign bodies (sutures) resolved the chronic infection. Chronic SSI can arise from underlying bacterial biofilms, which can invest implanted foreign bodies and associated soft tissue surfaces.

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Collaboration types
Domestic collaboration
Web of Science research areas
Infectious Diseases
Surgery
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