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Chronic testicular Chlamydia muridarum infection impairs mouse fertility and offspring development
Journal article   Open access   Peer reviewed

Chronic testicular Chlamydia muridarum infection impairs mouse fertility and offspring development

Emily R. Bryan, Kate A. Redgrove, Alison R. Mooney, Bettina P. Mihalas, Jessie M. Sutherland, Alison J. Carey, Charles W. Armitage, Logan K. Trim, Avinash Kollipara, Peter B. M. Mulvey, …
Biology of reproduction, v 102(4), pp 888-901
01 Apr 2020
PMID: 31965142
url
https://academic.oup.com/biolreprod/article-pdf/102/4/888/32994400/ioz229.pdfView
Published, Version of Record (VoR) Open
url
https://doi.org/10.1093/biolre/ioz229View
Published, Version of Record (VoR) Open

Abstract

Life Sciences & Biomedicine Reproductive Biology Science & Technology
With approximately 131 million new genital tract infections occurring each year, Chlamydia is the most common sexually transmitted bacterial pathogen worldwide. Male and female infections occur at similar rates and both cause serious pathological sequelae. Despite this, the impact of chlamydial infection on male fertility has long been debated, and the effects of paternal chlamydial infection on offspring development are unknown. Using a male mouse chronic infection model, we show that chlamydial infection persists in the testes, adversely affecting the testicular environment. Infection increased leukocyte infiltration, disrupted the blood:testis barrier and reduced spermiogenic cell numbers and seminiferous tubule volume. Sperm from infected mice had decreased motility, increased abnormal morphology, decreased zona-binding capacity, and increased DNA damage. Serum anti-sperm antibodies were also increased. When both acutely and chronically infected male mice were bred with healthy female mice, 16.7% of pups displayed developmental abnormalities. Female offspring of chronically infected sires had smaller reproductive tracts than offspring of noninfected sires. The male pups of infected sires displayed delayed testicular development, with abnormalities in sperm vitality, motility, and sperm-oocyte binding evident at sexual maturity. These data suggest that chronic testicular Chlamydia infection can contribute to male infertility, which may have an intergenerational impact on sperm quality. Summary sentence Testicular C. muridarum infection results in tissue damage and poor sperm quality and gives rise to offspring with serious abnormalities and developmental delays.

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Domestic collaboration
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Web of Science research areas
Reproductive Biology
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