Journal article
Circular dichroism and raman studies of the allosteric transition in methemoglobin
Biochemical and biophysical research communications, v 66(1), pp 444-450
1975
PMID: 240365
Abstract
Circular Dichroism and resonant Raman spectra have been measured for methemoglobin with and without inositol hexaphosphate (IHP) between pH 5 and pH 9. The transition induced by IHP is accompanied by large changes in the rotational strength of the Soret band. Without IHP, the rotational strength of methemoglobin euqals that of oxyhemoglobin; with IHP it equals that of deoxyhemoglobin. The rotational strength of the 260 nm band shows no change with IHP; the Raman bands change in a manner indicative of a shift in spin state equilibrium. The most direct explanation of these results is that IHP changes quaternary structure with few tertiary changes. It is proposed that the Soret rotational strength is a quantitative marker for quaternary structure.
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Details
- Title
- Circular dichroism and raman studies of the allosteric transition in methemoglobin
- Creators
- F.A Ferrone - Department of Chemistry Princeton University Princeton, New Jersey 08540, USAW.C Topp - Department of Chemistry Princeton University Princeton, New Jersey 08540, USA
- Publication Details
- Biochemical and biophysical research communications, v 66(1), pp 444-450
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Physics
- Web of Science ID
- WOS:A1975AN70600063
- Scopus ID
- 2-s2.0-0016701221
- Other Identifier
- 991014878538204721