Files and links (2)
Published, Version of Record (VoR)CC BY-NC-ND V4.0, Open
Journal article
Circulating microRNAs from the mouse tibia fracture model reflect the signature from patients with complex regional pain syndrome
Pain reports, v 6(3), pp e950-e950
01 Sep 2021
PMID: 34514274
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Introduction: Complex regional pain syndrome (CRPS) often results from an initial trauma that later produces a disproportionate amount of pain. The mechanisms underlying CRPS have been studied using a tibia fracture model (TFM) in rodents because this model closely mimics symptoms and has several molecular correlates observed in patients with CRPS. Objective: Here, we determined whether the TFM has alterations in circulating microRNAs (miRNAs) and cytokines transported by small extracellular vesicles (sEVs) that faithfully model previously reported miRNA alterations from patients with CRPS. Methods: We isolated and characterized serum-derived sEVs from mice 3 weeks after fracture when symptoms such as pain hypersensitivity develop. Whole-transcriptome profiling was used to determine sEV miRNAs, and Bio-Plex Pro Mouse Cytokine 23-plex assay was used to measure cytokines. Differentially expressed miRNAs from TFM were compared with previously reported circulating miRNA alterations from patients with CRPS. Results: Although sEV cytokine levels were unchanged, there were significant changes in sEV miRNA profiles. Differentially expressed miRNAs from TFM sEVs significantly overlapped with those previously reported in patients with CRPS. Of the 57 sEV miRNAs dysregulated in the TFM, 30 were previously reported in patients with CRPS compared with healthy control donors both in sEVs and 23 in whole blood. Conclusions: These findings enhance the validity of TFM as a model for CRPS and suggest that specific miRNA dysregulation may be a shared feature of CRPS and the TFM. These dysregulated miRNAs could help identify mechanistic targets or serve as biomarker candidates for both diagnosis and treatment responses in clinical trials.
Metrics
Details
- Title
- Circulating microRNAs from the mouse tibia fracture model reflect the signature from patients with complex regional pain syndrome
- Creators
- Jason R. Wickman - Drexel UniversityXuan Luo - Drexel UniversityWenwu Li - Vet Affairs Palo Alto Hlth Care Syst, Anesthesiol Serv, Palo Alto, CA USARenee Jean-Toussaint - Drexel UniversityPeyman Sahbaie - VA Palo Alto Health Care SystemAhmet Sacan - Drexel UniversityJ. David Clark - VA Palo Alto Health Care SystemSeena K. Ajit - Drexel University
- Publication Details
- Pain reports, v 6(3), pp e950-e950
- Publisher
- Lippincott Williams & Wilkins
- Number of pages
- 12
- Grant note
- R01NS102836; R01NS117340 / NIH NINDS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Neurological Disorders & Stroke (NINDS) I01RX001475 / Department of Veterans Affairs; US Department of Veterans Affairs
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pharmacology and Physiology; School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000711754400010
- Scopus ID
- 2-s2.0-85137411039
- Other Identifier
- 991019168400404721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Neurosciences