Journal article
Class III beta-tubulin is constitutively coexpressed with glial fibrillary acidic protein and nestin in midgestational human fetal astrocytes: Implications for phenotypic identity
Journal of neuropathology and experimental neurology, v 67(4), pp 341-354
01 Apr 2008
PMID: 18379434
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Class III beta-tubulin isotype (beta III-tubulin) is widely regarded as a neuronal marker in developmental neurobiology and stem cell research. To test the specificity of this marker protein, we determined its expression and distribution in primary cultures of glial fibrillary acidic protein (GFAP)-expressing astrocytes isolated from the cerebral hemispheres of 2 human fetuses at 18 to 20 weeks of gestation. Cells were maintained as monolayer cultures for 1 to 21 days without differentiation induction. By immunofluorescence microscopy, coexpression of beta III-tubulin and GFAP was detected in cells at all time points but in spatially distinct patterns. The numbers of GFAP(+) cells gradually decreased from Days 1 to 21 in vitro, whereas beta III-tubulin immunoreactivity was present in 100% of cells at all time points. beta-III-tubulin mRNA and protein expression were demonstrated in cultured cells by reverse-transcriptase-polymerase chain reaction and immunoblotting, respectively. Glial fibrillary acidic protein(+)/beta-III-tubulin-positive cells coexpressed nestin and vimentin but lacked neurofilament proteins, CD133, and glutamate-aspartate transporter. Weak cytoplasmic staining was detected with antibodies against microtubule-associated protein 2 isoforms. Confocal microscopy, performed on autopsy brain samples of human fetuses at 16 to 20 gestational weeks, revealed widespread colocalization of GFAP and beta III-tubulin in cells of the ventricular/subventricular zones and the cortical plate. Our results indicate that in the midgestational human brain, beta III-tubulin is not neuron specific because it is constitutively expressed in GFAP(+)/nestin(+) presumptive fetal astrocytes.
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Details
- Title
- Class III beta-tubulin is constitutively coexpressed with glial fibrillary acidic protein and nestin in midgestational human fetal astrocytes: Implications for phenotypic identity
- Creators
- Eduarda Draberova - Cytoskeleton (United States)Luis Del Valle - St. Christopher's Hospital for ChildrenJennifer Gordon - St. Christopher's Hospital for ChildrenVladimira Markova - Cytoskeleton (United States)Barbora Smejkalova - Cytoskeleton (United States)Louise Bertrand - St. Christopher's Hospital for ChildrenJean-Pierre de Chadarevian - St. Christopher's Hospital for ChildrenDimitri P. Agamanolis - Northeast Ohio Medical UniversityAgustin Legido - St. Christopher's Hospital for ChildrenKamel Khalili - St. Christopher's Hospital for ChildrenPavel Draber - Cytoskeleton (United States)Christos D. Katsetos - Akron Children's Hospital
- Publication Details
- Journal of neuropathology and experimental neurology, v 67(4), pp 341-354
- Publisher
- Oxford Univ Press
- Number of pages
- 14
- Grant note
- P01 NS30916 / NINDS NIH HHS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Neurological Disorders & Stroke (NINDS) P01NS030916 / NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Neurological Disorders & Stroke (NINDS)
- Resource Type
- Journal article
- Language
- English
- Web of Science ID
- WOS:000254589900008
- Scopus ID
- 2-s2.0-41949085038
- Other Identifier
- 991019353630104721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Clinical Neurology
- Neurosciences
- Pathology