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Clinical Significance of Flow Cytometric Deoxyribonucleic Acid Measurements of Deparaffinized Specimens in Bladder Tumors
Journal article   Peer reviewed

Clinical Significance of Flow Cytometric Deoxyribonucleic Acid Measurements of Deparaffinized Specimens in Bladder Tumors

Miao (a) Tingjie, Wang Ze, Xiong Rucheng and Cao Shilong
European urology, v 21(2), pp 98-120
1992
PMID: 1499623

Abstract

Clinical Paper
A retrospective study of flow cytometric measurements on paraffin-embedded tumor specimens from 188 patients with bladder tumor was conducted. Results were analyzed in combination with the morphological variation in bladder tumors. It was found that the deoxyribonucleic acid (DNA) ploidy pattern, the degree of infiltration and the multiplicity of bladder tumor were closely related with tumor recurrence, among which the DNA ploidy pattern was most significant. In aneuploid bladder tumors, the recurrence rate and the mean annual recurrence frequency were 76.7% and 1.46, respectively, and in the diploid bladder tumors, they were 18.7% and 0.33, respectively. Aneu- ploidy was the most indicative parameter of recurrence in bladder tumors. In addition, according to the DNA content and the ploidy level of the aneuploid cell lines, the aneuploid tumors in our group were divided into four types, namely tetraploid tumors, noneuploid tumors with an DNA index (DI) <1.5, hypotetraploid tumors and aneuploid tumors with several cell lines. The results showed that the recurrence rate of tetraploid tumors was relatively lower, and it became higher and higher in the following order: noneuploid tumors with DI < 1.5, noneuploid tumors with DI > 1.5, and two-aneuploid tumors. This indicates that there are different biological behaviors in tumors with different ploidy patterns. Finally, the correlation between the DNA ploidy pattern and tumor métastasés was also discussed.

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Web of Science research areas
Urology & Nephrology
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