Journal article
Clinical Significance of Serum COL6A3 in Pancreatic Ductal Adenocarcinoma
Journal of gastrointestinal surgery, v 18(1), pp 7-15
01 Jan 2014
PMID: 24002763
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Type VI collagen (COL6) forms a microfibrillar network often associated with type I collagen and constitutes a major component of the desmoplastic reaction in pancreatic ductal adenocarcinoma (PDA). We have demonstrated recently that the alpha 3 chain of COL6, COL6A3, is highly expressed in PDA tissue and undergoes tumor-specific alternative splicing. In this study, we investigated the diagnostic value and clinical significance of circulating COL6A3 protein and mRNA in PDA. COL6A3 levels in sera from patients with PDA (n = 44), benign lesions (n = 46) and age-matched healthy volunteers (n = 30) were analyzed by enzyme-linked immunosorbent assays (ELISA). Predictive abilities of COL6A3 were examined using receiver operating characteristic (ROC) curves from logistic regression models for PDA versus normal or benign serum levels. Expression levels were correlated with clinicopathological parameters. Real-time PCR was used to analyze the presence of COL6A3 mRNA containing alternative spliced exons E3, E4, and E6. Circulating COL6A3 protein levels were significantly elevated in PDA patients when compared to healthy sera (p = 0.0001) and benign lesions (p = 0.0035). The overall area under the ROC was 0.975. Log(COL6A3) alone provided good discrimination between PDA and benign lesions (area under the curve (AUC) = 0.817), but combined with CA19-9 provided excellent discrimination (AUC = 0.904). Interestingly, high COL6A3 serum levels were significantly associated with perineural invasion and cigarette smoking. Combined E3, E4, and E6 serum RNA values provided good sensitivity but low specificity. Our data demonstrate for the first time the potential clinical significance of circulating COL6A3 in the diagnosis of pancreatic malignancy.
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Details
- Title
- Clinical Significance of Serum COL6A3 in Pancreatic Ductal Adenocarcinoma
- Creators
- Christopher Y. Kang - Thomas Jefferson UniversityJonathan Wang - Thomas Jefferson UniversityDierdre Axell-House - Thomas Jefferson UniversityPranay Soni - Thomas Jefferson UniversityMon-Li Chu - Thomas Jefferson UniversityGalina Chipitsyna - Thomas Jefferson UniversityKonrad Sarosiek - Thomas Jefferson UniversityJocelyn Sendecki - Thomas Jefferson UniversityTerry Hyslop - Thomas Jefferson UniversityMazhar Al-Zoubi - Thomas Jefferson UniversityCharles J. Yeo - Thomas Jefferson UniversityHwyda A. Arafat - Thomas Jefferson University
- Publication Details
- Journal of gastrointestinal surgery, v 18(1), pp 7-15
- Publisher
- Springer Nature
- Number of pages
- 9
- Grant note
- R21 CA133753 / NCI NIH HHS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- College of Medicine
- Web of Science ID
- WOS:000329405400002
- Scopus ID
- 2-s2.0-84891859567
- Other Identifier
- 991022047280804721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Gastroenterology & Hepatology
- Surgery