Journal article
Cloning, Characterization, and Expression of a Calcitonin Receptor from Guinea Pig Brain
Journal of neurochemistry, v 69(2), pp 455-464
Aug 1997
PMID: 9231703
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
: A calcitonin receptor was cloned from guinea pig brain by using a degenerate reverse transcription‐polymerase chain reaction (RT‐PCR) strategy. When the cloned guinea pig calcitonin receptor was transfected into COS 1 cells, salmon calcitonin stimulated intracellular cyclic AMP accumulation with an EC50 of 0.1 nM, whereas human calcitonin was >250‐fold less potent (EC50 27.6 nM). Related neuropeptides rat αCGRP and rat amylin did not activate the guinea pig calcitonin receptor at physiologic concentrations. Stimulation of the transfected guinea pig calcitonin receptor by salmon calcitonin also resulted in phosphatidylinositol hydrolysis with an EC50 of 2.5 nM. Expression of the calcitonin receptor was mapped by a combination of RT‐PCR, northern analysis, and expression in Xenopus oocytes. The guinea pig calcitonin receptor was most highly expressed in diencephalon and a single subtype was detected.
Metrics
Details
- Title
- Cloning, Characterization, and Expression of a Calcitonin Receptor from Guinea Pig Brain
- Creators
- Atom Sarkar - University of MiamiIan M. Dickerson - University of Miami
- Publication Details
- Journal of neurochemistry, v 69(2), pp 455-464
- Publisher
- Blackwell Science Ltd
- Number of pages
- 10
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurology; Neurosurgery
- Web of Science ID
- WOS:A1997XL15400002
- Scopus ID
- 2-s2.0-0030838494
- Other Identifier
- 991021960654004721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemistry & Molecular Biology
- Neurosciences