Journal article
Cloning and expression of rabbit CCT subunits eta and beta in healing cutaneous wounds
Cell stress & chaperones, v 15(6), pp 819-826
Nov 2010
PMID: 20393890
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
We have previously identified the CCT subunit eta as specifically reduced in healing fetal skin wounds by differential display, and observed that this reduction is not seen with any other CCT subunit. We now report the cloning and characterization of the cDNAs for rabbit CCT-eta and its closest evolutionary homolog, CCT-beta. Quantitative examination of CCT-eta and –beta message expression in healing fetal and adult wounds at 12 h post-injury confirms that CCT-eta mRNA is decreased in fetal wound tissues, but actually elevated in adult wound tissues. CCT-beta mRNA, in contrast, remains unchanged in both fetal and adult wound tissues. CCT-eta mRNA remains persistently elevated in healing adult wounds for 28 days following injury, whereas CCT-beta mRNA remains invariant throughout. CCT-eta protein is similarly increased, whereas CCT-beta protein remains unchanged.
-smooth muscle actin (
-SMA), a recognized substrate of CCT known to be important in integumentary wound healing, was also measured over the course of wound healing, and both mRNA and protein levels were elevated throughout the 28 days.
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Details
- Title
- Cloning and expression of rabbit CCT subunits eta and beta in healing cutaneous wounds
- Creators
- Latha Satish - Center for Genomic Sciences, Allegheny-Singer Research Institute, Allegheny General Hospital, Pittsburgh, PA 15212 USASandra Johnson - Center for Genomic Sciences, Allegheny-Singer Research Institute, Allegheny General Hospital, Pittsburgh, PA 15212 USAAdam Abdulally - Center for Genomic Sciences, Allegheny-Singer Research Institute, Allegheny General Hospital, Pittsburgh, PA 15212 USAJ. Christopher Post - Center for Genomic Sciences, Allegheny-Singer Research Institute, Allegheny General Hospital, Pittsburgh, PA 15212 USAGarth D Ehrlich - Center for Genomic Sciences, Allegheny-Singer Research Institute, Allegheny General Hospital, Pittsburgh, PA 15212 USASandeep Kathju - Center for Genomic Sciences, Allegheny-Singer Research Institute, Allegheny General Hospital, Pittsburgh, PA 15212 USA
- Publication Details
- Cell stress & chaperones, v 15(6), pp 819-826
- Publisher
- Springer Netherlands; Dordrecht
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000282971000006
- Scopus ID
- 2-s2.0-78149466034
- Other Identifier
- 991014877701104721
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- Web of Science research areas
- Cell Biology