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Cloning of the GATA-binding protein that regulates endothelin-1 gene expression in endothelial cells
Journal article   Open access   Peer reviewed

Cloning of the GATA-binding protein that regulates endothelin-1 gene expression in endothelial cells

M E Lee, D H Temizer, J A Clifford and T Quertermous
The Journal of biological chemistry, v 266(24), pp 16188-16192
25 Aug 1991
DOI: https://doi.org/10.1016/S0021-9258(18)98533-9
PMID: 1714909
Featured in Collection :   UN Sustainable Development Goals @ Drexel
url
https://doi.org/10.1016/s0021-9258(18)98533-9View
Published, Version of Record (VoR) Open
url
https://doi.org/10.1016/S0021-9258(18)98533-9View
Published, Version of Record (VoR) Open

Abstract

Amino Acid Sequence Animals Base Sequence Blotting, Northern Cells, Cultured Chickens Cloning, Molecular DNA - genetics DNA-Binding Proteins - genetics DNA-Binding Proteins - physiology Endothelins - genetics Endothelium, Vascular - metabolism GATA2 Transcription Factor Gene Expression Regulation HeLa Cells Humans Molecular Sequence Data RNA - genetics Sequence Homology, Nucleic Acid Transcription Factors - genetics Transcription Factors - physiology Transcriptional Activation Tumor Cells, Cultured
Previously, we have identified two regions (A and B) of the endothelin-1 promoter that are important for the expression of this gene in cultured vascular endothelial cells. The cis-acting sequence in one of these regions (Region A) includes the core binding motif GATA, raising the possibility that this region of DNA mediates binding of a member of the GATA-binding protein family. In this report, we describe the use of polymerase chain reaction in conjunction with cDNA cloning to characterize the GATA-binding protein expressed in endothelial cells. The nucleotide sequence of endothelial cell cDNA clones is highly homologous to that of the chicken GATA-2 (NF-E1b) gene, indicating that our clones encode the human GATA-2 gene transcript. By RNA blot analysis, this gene is expressed in cultured cell lines derived from a number of different tissues. Transactivation experiments utilizing human GATA-2 eukaryotic expression vectors indicate that the GATA-2 protein interacts with the endothelin-1 GATA sequence to increase transcription of reporter genes in both BAEC and HeLa cells. These data provide the first evidence for a non-erythroid target gene regulated by GATA-2 and indicate that GATA-2 may have a more broad role in transcriptional regulation than the erythroid-specific GATA-1 protein.

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Web of Science research areas
Biochemistry & Molecular Biology
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