Journal article
Coexistence of Native-Like and Non-Native Cytochrome c on Anionic Liposomes with Different Cardiolipin Content
The journal of physical chemistry. B, v 119(40), pp 12846-12859
08 Oct 2015
PMID: 26369421
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
We employed a combination of fluorescence, visible circular dichroism, and absorption spectroscopy to study the conformational changes of ferricytochrome c upon its binding to cardiolipin-containing small unilamellar vesicles. The measurements were performed as a function of the cardiolipin concentration, the cardiolipin content of the liposomes, and the NaCl concentration of the solvent. The data were analyzed with a novel model that combines a single binding step with a conformational equilibrium between native-like and non-native-like proteins bound to the membrane surface. The equilibrium between the two conformations, which themselves are comprised of structurally slightly different subconformations, shifts to the more non-native-like conformation with increasing cardiolipin concentration. For the binding isotherms described in this paper, we explicitly considered the enthalpic and entropic contributions of molecular crowding to protein binding at low lipid concentrations and high occupancy of the liposome surface. Increasing the CL content of liposomes increases the overall binding affinity but makes the conformational distribution much more susceptible to the influence of sodium and chloride ions, which shifts the equilibrium toward the more native-like state and directly inhibits binding, particularly to liposomes with 100% cardiolipin content. Spectroscopic evidence further suggests that a fraction of the non-native conformers adopts a pentacoordinated state similar to those obtained in class C peroxidases. On the basis of our results, we propose a hypothesis that describes the balance between facilitating and impeding forces controlling the peroxidase activity of cytochrome c in the inner membrane space of mitochondria.
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Details
- Title
- Coexistence of Native-Like and Non-Native Cytochrome c on Anionic Liposomes with Different Cardiolipin Content
- Creators
- Leah A. Pandiscia - Drexel UniversityReinhard Schweitzer-Stenner - Drexel University
- Publication Details
- The journal of physical chemistry. B, v 119(40), pp 12846-12859
- Publisher
- American Chemical Society; Washington, DC
- Number of pages
- 14
- Grant note
- Department of Chemistry
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- [Retired Faculty]
- Web of Science ID
- WOS:000362701900012
- Scopus ID
- 2-s2.0-84944050949
- Other Identifier
- 991019167749704721
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InCites Highlights
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- Web of Science research areas
- Chemistry, Physical