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Cold Plasma Inactivation of Bacillus cereus and Bacillus anthracis (Anthrax) Spores
Journal article

Cold Plasma Inactivation of Bacillus cereus and Bacillus anthracis (Anthrax) Spores

Danil Dobrynin, Gregory Fridman, Yurii V Mukhin, Meghan A Wynosky-Dolfi, Judy Rieger, Richard F Rest, Alexander F Gutsol and Alexander Fridman
IEEE transactions on plasma science, v 38(8), pp 1878-1884
Aug 2010

Abstract

Plasma applications Bacillus anthracis Surface discharges Surface morphology Dielectrics Mechanical engineering spore inactivation Fungi Atmospheric-pressure plasmas Microorganisms Anthrax sterilization atmospheric-pressure dielectric barrier discharge (DBD) nonequilibrium plasma nonthermal plasma Biomedical engineering Immune system
Bacillus spores represent one of the most resistant organisms to conventional sterilization methods. This paper is focused on the inactivation of the spores of two Bacillus species, Bacillus cereus and Bacillus anthracis, using atmospheric-pressure dielectric-barrier-discharge (DBD) plasma. Spores treated in liquid or air-dried on a solid surface were effectively inactivated within 1 min of DBD plasma treatment at a discharge power of 0.3 W/cm 2 . Results of a series of model experiments show that neutral reactive oxygen species and UV radiation play a dominant role in the inactivation of spores. We also show that 45 s of the DBD plasma treatment of air-dried spores placed inside closed plastic or paper envelopes permits up to 7 log reduction of viable spores.

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Web of Science research areas
Physics, Fluids & Plasmas
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