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Colocalization of the B cell receptor and CD20 followed by activation-dependent dissociation in distinct lipid rafts
Journal article   Open access   Peer reviewed

Colocalization of the B cell receptor and CD20 followed by activation-dependent dissociation in distinct lipid rafts

Ryan J Petrie and Julie P Deans
The Journal of immunology (1950), v 169(6), pp 2886-2891
15 Sep 2002
DOI: https://doi.org/10.4049/jimmunol.169.6.2886
PMID: 12218101
Featured in Collection :   UN Sustainable Development Goals @ Drexel
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http://www.jimmunol.org/content/169/6/2886.full.pdfView
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Abstract

Antigens, CD20 - immunology Antigens, CD20 - metabolism B-Lymphocytes - drug effects B-Lymphocytes - immunology B-Lymphocytes - metabolism beta-Cyclodextrins Cell Membrane - immunology Cell Membrane - metabolism Cyclodextrins - pharmacology Detergents - pharmacology Endocytosis - drug effects Endocytosis - immunology Humans Kinetics Lymphocyte Activation Membrane Microdomains - drug effects Membrane Microdomains - immunology Membrane Microdomains - metabolism Microscopy, Fluorescence Receptors, Antigen, B-Cell - antagonists & inhibitors Receptors, Antigen, B-Cell - immunology Receptors, Antigen, B-Cell - metabolism Tumor Cells, Cultured
The B cell Ag receptor (BCR) and CD20, a putative calcium channel, inducibly associate with cholesterol-dependent membrane microdomains known as lipid rafts. A functional association between the BCR and CD20 is suggested by the effects of CD20-specific mAbs, which can modulate cell cycle transitions elicited by BCR signaling. Using immunofluorescence microscopy we show here that the BCR and CD20 colocalize after receptor ligation and then rapidly dissociate at the cell surface before endocytosis of the BCR. After separation, surface BCR and CD20 were detected in distinct lipid rafts isolated as low density, detergent-resistant membrane fragments. Pretreatment with methyl-beta-cyclodextrin, which we have previously shown to enhance receptor-mediated calcium mobilization, did not prevent colocalization of the BCR and CD20, but slowed their dissociation. The data demonstrate rapid dynamics of the BCR in relation to CD20 at the cell surface. Activation-dependent dissociation of the BCR from CD20 occurs before receptor endocytosis and appears to require in part the integrity of lipid rafts.

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Web of Science research areas
Immunology
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