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Journal article
Comapping Cellular Content and Extracellular Matrix with Hemodynamics in Intact Arterial Tissues Using Scanning Immunofluorescent Multiphoton Microscopy
Microscopy and microanalysis, v 30(2), pp 342-358
29 Apr 2024
PMID: 38525887
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Deviation of blood flow from an optimal range is known to be associated with the initiation and progression of vascular pathologies. Important open questions remain about how the abnormal flow drives specific wall changes in pathologies such as cerebral aneurysms where the flow is highly heterogeneous and complex. This knowledge gap precludes the clinical use of readily available flow data to predict outcomes and improve treatment of these diseases. As both flow and the pathological wall changes are spatially heterogeneous, a crucial requirement for progress in this area is a methodology for acquiring and comapping local vascular wall biology data with local hemodynamic data. Here, we developed an imaging pipeline to address this pressing need. A protocol that employs scanning multiphoton microscopy was developed to obtain three-dimensional (3D) datasets for smooth muscle actin, collagen, and elastin in intact vascular specimens. A cluster analysis was introduced to objectively categorize the smooth muscle cells (SMC) across the vascular specimen based on SMC actin density. Finally, direct quantitative comparison of local flow and wall biology in 3D intact specimens was achieved by comapping both heterogeneous SMC data and wall thickness to patient-specific hemodynamic results.
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- Title
- Comapping Cellular Content and Extracellular Matrix with Hemodynamics in Intact Arterial Tissues Using Scanning Immunofluorescent Multiphoton Microscopy
- Creators
- Yasutaka Tobe (Corresponding Author) - University of PittsburghAnne M. Robertson - University of PittsburghMehdi Ramezanpour - University of PittsburghJuan R. Cebral - George Mason UniversitySimon C. Watkins - University of PittsburghFady T. Charbel - University of Illinois ChicagoSepideh Amin-Hanjani - College Station Medical CenterAlexander K. Yu - Allegheny Health NetworkBoyle C. Cheng - Allegheny Health NetworkHenry H. Woo - Donald & Barbara Zucker School of Medicine at Hofstra/Northwell
- Publication Details
- Microscopy and microanalysis, v 30(2), pp 342-358
- Publisher
- Oxford Univ Press
- Number of pages
- 17
- Grant note
- University of Pittsburgh National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Surgery; Neurosurgery
- Web of Science ID
- WOS:001189941600001
- Scopus ID
- 2-s2.0-85191898751
- Other Identifier
- 991021930318604721
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InCites Highlights
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Materials Science, Multidisciplinary
- Microscopy