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Preprint (Author's original)CC BY-NC-ND V4.0, Open
Journal article
Combination of Physicochemical Tropism and Affinity Moiety Targeting of Lipid Nanoparticles Enhances Organ Targeting
Nano letters, v 24(16), pp 4774-4784
10 Apr 2024
PMID: 38598417
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Two camps have emerged for targeting nanoparticles to specific organs and cell types: affinity moiety targeting and physicochemical tropism. Here we directly compare and combine both using intravenous (IV) lipid nanoparticles (LNPs) designed to target the lungs. We utilized PECAM antibodies as affinity moieties and cationic lipids for physicochemical tropism. These methods yield nearly identical lung uptake, but aPECAM LNPs show higher endothelial specificity. LNPs combining these targeting methods had >2-fold higher lung uptake than either method alone and markedly enhanced epithelial uptake. To determine if lung uptake is because the lungs are the first organ downstream of IV injection, we compared IV vs intra-arterial (IA) injection into the carotid artery, finding that IA combined-targeting LNPs achieve 35% of the injected dose per gram (%ID/g) in the first-pass organ, the brain, among the highest reported. Thus, combining the affinity moiety and physicochemical strategies provides benefits that neither targeting method achieves alone.
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Details
- Title
- Combination of Physicochemical Tropism and Affinity Moiety Targeting of Lipid Nanoparticles Enhances Organ Targeting
- Creators
- Marco E Zamora - Drexel UniversitySerena Omo-Lamai - University of PennsylvaniaManthan N Patel - University of PennsylvaniaJichuan Wu - University of PennsylvaniaEvguenia Arguiri - Raymond and Ruth Perelman School of Medicine at the University of PennsylvaniaVladmir R Muzykantov - University of PennsylvaniaJacob W Myerson (Corresponding Author) - Raymond and Ruth Perelman School of Medicine at the University of PennsylvaniaOscar A Marcos-Contreras (Corresponding Author) - University of PennsylvaniaJacob S Brenner (Corresponding Author) - Raymond and Ruth Perelman School of Medicine at the University of Pennsylvania
- Publication Details
- Nano letters, v 24(16), pp 4774-4784
- Publisher
- American Chemical Society
- Number of pages
- 11
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:001200233600001
- Scopus ID
- 2-s2.0-85190115316
- Other Identifier
- 991021867242204721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Chemistry, Multidisciplinary
- Chemistry, Physical
- Materials Science, Multidisciplinary
- Nanoscience & Nanotechnology
- Physics, Applied
- Physics, Condensed Matter