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Accepted (AM)Open Access (License Unspecified), Open
Journal article
Combining Laser Capture Microdissection and Microfluidic qPCR to Analyze Transcriptional Profiles of Single Cells: A Systems Biology Approach to Opioid Dependence
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, v 2020(157)
01 Mar 2020
PMID: 32202523
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Profound transcriptional heterogeneity in anatomically adjacent single cells suggests that robust tissue functionality may be achieved by cellular phenotype diversity. Single-cell experiments investigating the network dynamics of biological systems demonstrate cellular and tissue responses to various conditions at biologically meaningful resolution. Herein, we explain our methods for gathering single cells from anatomically specific locations and accurately measuring a subset of their gene expression profiles. We combine laser capture microdissection (LCM) with microfluidic reverse transcription quantitative polymerase chain reactions (RT-qPCR). We also use this microfluidic RT-qPCR platform to measure the microbial abundance of gut contents.
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Details
- Title
- Combining Laser Capture Microdissection and Microfluidic qPCR to Analyze Transcriptional Profiles of Single Cells: A Systems Biology Approach to Opioid Dependence
- Creators
- Sean J. O'Sullivan - Thomas Jefferson Univ, Dept Pathol, Daniel Baugh Inst Funct Genom & Computat Biol, Philadelphia, PA 19107 USABeverly A. S. Reyes - Drexel UniversityRajanikanth Vadigepalli - Thomas Jefferson UniversityElisabeth J. Van Bockstaele - Drexel UniversityJames S. Schwaber - Thomas Jefferson University
- Publication Details
- JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, v 2020(157)
- Publisher
- Journal Of Visualized Experiments
- Number of pages
- 8
- Grant note
- HLB U01 HL133360 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA R21 DA036372; T32 AA-007463 / NIDA; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute on Drug Abuse (NIDA)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pharmacology and Physiology
- Web of Science ID
- WOS:000523286100036
- Scopus ID
- 2-s2.0-85081544792
- Other Identifier
- 991019184825904721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biotechnology & Applied Microbiology