Journal article
Combining Peripheral Nerve Grafting and Matrix Modulation to Repair the Injured Rat Spinal Cord
Journal of Visualized Experiments, (33)
2009
PMID: 19935638
Abstract
Traumatic injury to the spinal cord (SCI) causes death of neurons, disruption of motor and sensory nerve fiber (axon) pathways and disruption of communication with the brain. One of the goals of our research is to promote axon regeneration to restore connectivity across the lesion site. To accomplish this we developed a peripheral nerve (PN) grafting technique where segments of sciatic nerve are either placed directly between the damaged ends of the spinal cord or are used to form a bridge across the lesion. There are several advantages to this approach compared to transplantation of other neural tissues; regenerating axons can be directed towards a specific target area, the number and source of regenerating axons is easily determined by tracing techniques, the graft can be used for electrophysiological experiments to measure functional recovery associated with axons in the graft, and it is possible to use an autologous nerve to reduce the possibility of graft rejection. In our lab we have performed both autologous (donor and recipient are the same animal) and heterologous (donor and recipient are different animals) grafts with comparable results. This approach has been used successfully in both acute and chronic injury situations. Regenerated axons that reach the distal end of the PN graft often fail to extend back into the spinal cord, so we use microinjections of chondroitinase to degrade inhibitory molecules associated with the scar tissue surrounding the area of SCI. At the same time we have found that providing exogenous growth and trophic molecules encourages longer distance axonal regrowth into the spinal cord. Several months after transplantation we perform a variety of anatomical, behavioral and electrophysiological tests to evaluate the recovery of function in our spinal cord injured animals. This experimental approach has been used successfully in several spinal cord injury models, at different levels of injury and in different species (mouse, rat and cat). Importantly, the peripheral nerve grafting approach is effective in promoting regeneration by acute and chronically injured neurons.
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19 citations in Scopus
Details
- Title
- Combining Peripheral Nerve Grafting and Matrix Modulation to Repair the Injured Rat Spinal Cord
- Creators
- John D Houle - Department of Neurobiology and Anatomy, Drexel University College of MedicineArthi Amin - Department of Neurobiology and Anatomy, Drexel University College of MedicineMarie-Pascale Cote - Department of Neurobiology and Anatomy, Drexel University College of MedicineMichel Lemay - Department of Neurobiology and Anatomy, Drexel University College of MedicineKassi Miller - Department of Neurobiology and Anatomy, Drexel University College of MedicineHarra Sandrow - Department of Neurobiology and Anatomy, Drexel University College of MedicineLauren Santi - Department of Neurobiology and Anatomy, Drexel University College of MedicineJed Shumsky - Department of Neurobiology and Anatomy, Drexel University College of MedicineVeronica Tom - Department of Neurobiology and Anatomy, Drexel University College of Medicine
- Publication Details
- Journal of Visualized Experiments, (33)
- Publisher
- MyJove Corporation
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Scopus ID
- 2-s2.0-77954041673
- Other Identifier
- 991014877714104721