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Journal article
Comparative Effects of Graphene and Molybdenum Disulfide on Human Macrophage Toxicity
Small (Weinheim an der Bergstrasse, Germany), v 16(35), 2002194
01 Sep 2020
PMID: 32743979
Abstract
Graphene and other 2D materials, such as molybdenum disulfide, have been increasingly used in electronics, composites, and biomedicine. In particular, MoS(2)and graphene hybrids have attracted a great interest for applications in the biomedical research, therefore stimulating a pertinent investigation on their safety in immune cells like macrophages, which commonly engulf these materials. In this study, M1 and M2 macrophage viability and activation are mainly found to be unaffected by few-layer graphene (FLG) and MoS(2)at doses up to 50 mu g mL(-1). The uptake of both materials is confirmed by transmission electron microscopy, inductively coupled plasma mass spectrometry, and inductively coupled plasma atomic emission spectroscopy. Notably, both 2D materials increase the secretion of inflammatory cytokines in M1 macrophages. At the highest dose, FLG decreases CD206 expression while MoS(2)decreases CD80 expression.CathBandCathLgene expressions are dose-dependently increased by both materials. Despite a minimal impact on the autophagic pathway, FLG is found to increase the expression ofAtg5and autophagic flux, as observed by Western blotting of LC3-II, in M1 macrophages. Overall, FLG and MoS(2)are of little toxicity in human macrophages even though they are found to trigger cell stress and inflammatory responses.
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Details
- Title
- Comparative Effects of Graphene and Molybdenum Disulfide on Human Macrophage Toxicity
- Creators
- Hazel Lin - Centre National de la Recherche ScientifiqueDing-Kun Ji - Univ Strasbourg, ISIS, Immunol Immunopathol & Therapeut Chem, CNRS,UPR 3572, F-67000 Strasbourg, FranceMatteo Andrea Lucherelli - Centre National de la Recherche ScientifiqueGiacomo Reina - Université de StrasbourgStefano Ippolito - Université de StrasbourgPaolo Samori - Univ Strasbourg, ISIS, CNRS, F-67000 Strasbourg, FranceAlberto Bianco - Université de Strasbourg
- Publication Details
- Small (Weinheim an der Bergstrasse, Germany), v 16(35), 2002194
- Publisher
- Wiley
- Number of pages
- 13
- Grant note
- ANR-11-EQPX-022 / Agence Nationale de la Recherche (ANR) through the program "Investissements d'Avenir"; Agence Nationale de la Recherche (ANR) ANR-10-LABX-0026_CSC / Agence Nationale de la Recherche (ANR) through the LabEx project Chemistry of Complex Systems; Agence Nationale de la Recherche (ANR) 785219 / EU Graphene Flagship project
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- A.J. Drexel Nanomaterials Institute
- Web of Science ID
- WOS:000554472400001
- Scopus ID
- 2-s2.0-85088825624
- Other Identifier
- 991021931767404721
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Chemistry, Multidisciplinary
- Chemistry, Physical
- Materials Science, Multidisciplinary
- Nanoscience & Nanotechnology
- Physics, Applied
- Physics, Condensed Matter